School of Chemistry and Chemical Engineering, Chongqing University of Science and Technology, Chongqing, 401331, China.
School of Chemistry and Chemical Engineering, Chongqing University of Science and Technology, Chongqing, 401331, China.
Talanta. 2025 Jan 1;281:126884. doi: 10.1016/j.talanta.2024.126884. Epub 2024 Sep 14.
Hexavalent chromium (Cr(VI)) is an environmental pollutant and recognized as a human carcinogen. Therefore, it is necessary to develop a simple and sensitive detection technique for Cr(VI). Herein, it is found that Cu interacts with guanosine 5'-monophosphate (GMP) to form a homogeneous Cu(II)-GMP complex (Cu·GMP) that efficiently displays the oxidoreductase-like catalytic activity. Cu·GMP can catalyze the oxidation between Cr(VI) and substrate 3,3',5,5'- tetramethylbenzidine (TMB), resulting in color change recognized by the naked eyes. Base on this, a convenient colorimetric assay for Cr(VI) detection was developed. The detection limit (3σ/s) of this sensor for Cr(VI) was 23 nM with a linear range of 0.1-25 μM. Moreover, the proposed assay was successfully applied to detect Cr(VI) in different environmental water samples with satisfactory recoveries. Our method is simple, efficient, rapid and cost-effective for Cr(VI) detection without the need for complicated material preparation or special separation, which shows great potential in environmental monitoring.
六价铬（Cr(VI)）是一种环境污染物，被认为是人类致癌物。因此，有必要开发一种简单、灵敏的 Cr(VI)检测技术。在此，我们发现 Cu 与鸟苷 5'-单磷酸（GMP）相互作用形成均相的 Cu(II)-GMP 配合物（Cu·GMP），该配合物具有高效的氧化还原酶样催化活性。Cu·GMP 能够催化 Cr(VI)与底物 3,3',5,5'-四甲基联苯胺（TMB）之间的氧化还原反应，从而产生肉眼可识别的颜色变化。基于此，我们开发了一种方便的比色法测定 Cr(VI)的方法。该传感器对 Cr(VI)的检测限（3σ/s）为 23 nM，线性范围为 0.1-25 μM。此外，该方法还成功地应用于不同环境水样中 Cr(VI)的检测，回收率令人满意。我们的方法简单、高效、快速且具有成本效益，无需复杂的材料制备或特殊分离，在环境监测方面具有很大的应用潜力。
