Henan Engineering Technology Research Center of Food Processing and Circulation Safety Control, College of Food Science and Technology, Henan Agricultural University, 63 Nongye Road, Zhengzhou, Henan, 450002, China.
College of Veterinary Medicine, Northwest A&F University, 22 Xinong Road, Yangling, 712100, China.
Biosens Bioelectron. 2025 Jan 1;267:116779. doi: 10.1016/j.bios.2024.116779. Epub 2024 Sep 11.
In this study, we incorporated nanometal surface energy transfer (NSET) in lateral flow immunoassay (LFIA) and explored the relationship between fluorescence quenching efficiency and detection sensitivity to improve sensitivity of NSET-LFIA system. We developed nine gold nanoparticles (GNPs) with absorption spectrum in the range of 520-605 nm as acceptors and quantum dot microspheres (QDMs) with emission spectrum of 530, 570, and 610 nm as donors. By analyzing the overlap integral area, fluorescence quenching efficiency, and detection sensitivity of 27 donor-acceptor pairs, we observed that the larger overlap integral area led to higher fluorescence quenching efficiency and detection sensitivity. A maximum fluorescence quenching efficiency of 91.0% was obtained from the combination of GNPs at 605 nm and QDMs at 610 nm, achieving the highest detection sensitivity. We developed NSET-LFIA for the detection of T2 toxin with a limit of detection of 0.04 ng/mL, which was 10-times higher than that obtained via conventional GNP-LFIA. NSET-LFIA represents a versatile, ultrasensitive and valuable screening tool for small molecules in real samples.
在这项研究中，我们将纳米金属表面能量转移（NSET）纳入侧向流动免疫分析（LFIA）中，并探索了荧光猝灭效率与检测灵敏度之间的关系，以提高 NSET-LFIA 系统的灵敏度。我们开发了九种金纳米粒子（GNPs），其吸收光谱范围为 520-605nm，作为受体，以及发射光谱为 530、570 和 610nm 的量子点微球（QDMs）作为供体。通过分析 27 对供体-受体对的重叠积分面积、荧光猝灭效率和检测灵敏度，我们观察到较大的重叠积分面积导致更高的荧光猝灭效率和检测灵敏度。从 605nm 的 GNPs 和 610nm 的 QDMs 的组合中获得了 91.0%的最大荧光猝灭效率，实现了最高的检测灵敏度。我们开发了用于检测 T2 毒素的 NSET-LFIA，检测限为 0.04ng/mL，比传统的 GNP-LFIA 高 10 倍。NSET-LFIA 代表了一种用于实际样品中小分子的多功能、超灵敏和有价值的筛选工具。
