Qi Houjuan, Yang Lifei, Ma Rongxiu, Xiang Yushuang, Dai Yuxin, Ren Juanna, Xu Ben Bin, El-Bahy Zeinhom M, Thabet Hamdy Khamees, Huang Zhanhua, Ben Wei, Yu Huimin, Guo Zhanhu
Key Laboratory of Bio-based Material Science & Technology, Material Science and Engineering College, Northeast Forestry University, Harbin 150040, China.
Mechanical and Construction Engineering, Faculty of Engineering and Environment, Northumbria University, Newcastle upon Tyne NE1 8ST, UK; School of Materials Science and Engineering, Taiyuan University of Science and Technology, Taiyuan 030024, China.
Int J Biol Macromol. 2024 Sep 15;280(Pt 1):135701. doi: 10.1016/j.ijbiomac.2024.135701.
Wound healing is a complex process and reuires a long repair process. Poor healing effect is normally a challenge for wound healing. Designing sponge dressings with drug-assisted therapy, good breathability, and multiple functional structures effectively promotes wound healing. In this work, a flexible amoxicillin-laded (AMX) sodium alginate (SA)/cellulose nanocrystals (CNCs)/ polyvinyl alcoho (PVA) (SA/CNCs/PVA-AMX, SCP-AMX) wound dressing was designed and built with an excellent porous structure, suitable porosity, and anti-bacterial properties for promoting wound tissue reparation. The porous structure of the wound dressing was fabricated by freeze-thawing cyclic and freeze-dried molding process. This wound dressing exhibited a 3D porous structure for soft-tissue-engineering application, including high porosity (84.2%), swelling ratio (1513%), tensile strength (1.79 MPA), and flexibility. With the inhibition zones of Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) being 1.96 and4.58 cm, respectively, this wound dressing demonstrated good antibacterial activity against E. coli and S. aureus. More importantly, wound healing assay in vivo indicates that SCP-AMX could inhibit wound infection, promote collagen deposition, reduce inflammation, and accelerate granulation tissue and wound healing. Thus, the reported wounding dressings present excellent biocompatibility, high antibacterial activities, and excellent biosafety with great potential in wound healing applications.
伤口愈合是一个复杂的过程，需要漫长的修复过程。愈合效果不佳通常是伤口愈合面临的一项挑战。设计具有药物辅助治疗、良好透气性和多种功能结构的海绵敷料可有效促进伤口愈合。在这项工作中，设计并构建了一种柔性载阿莫西林（AMX）的海藻酸钠（SA）/纤维素纳米晶体（CNCs）/聚乙烯醇（PVA）（SA/CNCs/PVA-AMX，SCP-AMX）伤口敷料，其具有优异的多孔结构、合适的孔隙率和抗菌性能，可促进伤口组织修复。伤口敷料的多孔结构通过冻融循环和冷冻干燥成型工艺制备。这种伤口敷料呈现出用于软组织工程应用的三维多孔结构，包括高孔隙率（84.2%）、膨胀率（1513%）、拉伸强度（1.79兆帕）和柔韧性。该伤口敷料对大肠杆菌和金黄色葡萄球菌的抑菌圈分别为1.96厘米和4.58厘米，表明其对大肠杆菌和金黄色葡萄球菌具有良好的抗菌活性。更重要的是，体内伤口愈合试验表明，SCP-AMX可抑制伤口感染、促进胶原蛋白沉积、减轻炎症并加速肉芽组织生长和伤口愈合。因此，所报道的伤口敷料具有优异的生物相容性、高抗菌活性和出色的生物安全性，在伤口愈合应用中具有巨大潜力。
