Luzhou Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, The Affiliated Stomatological Hospital of Southwest Medical University, Luzhou 646000, China.
Luzhou Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, The Affiliated Stomatological Hospital of Southwest Medical University, Luzhou 646000, China; Department of Oral and Maxillofacial Surgery, The Affiliated Stomatological Hospital of Southwest Medical University, Luzhou 646000, China; School of Stomatology, Southwest Medical University, Luzhou 646000, China.
Int J Biol Macromol. 2023 May 15;237:123944. doi: 10.1016/j.ijbiomac.2023.123944. Epub 2023 Mar 9.
An ideal wound dressing should have excellent antimicrobial properties and provide a suitable microenvironment for regenerating damaged skin tissue. In this study, we utilized sericin to biosynthesize silver nanoparticles in situ and introduced curcumin to obtain Sericin-AgNPs/Curcumin (Se-Ag/Cur) antimicrobial agent. The hybrid antimicrobial agent was then encapsulated in a physically double cross-linking 3D structure network (Sodium alginate-Chitosan, SC) to obtain the SC/Se-Ag/Cur composite sponge. The 3D structural networks were constructed through electrostatic interactions between sodium alginate and chitosan and ionic interactions between sodium alginate and calcium ions. The prepared composite sponges have excellent hygroscopicity (contact angle 51.3° ± 5.6°), moisture retention ability, porosity (67.32 % ± 3.37 %), and mechanical properties (>0.7 MPa) and exhibit good antibacterial ability against Pseudomonas aeruginosa (P. aeruginosa) and Staphylococcus aureus (S. aureus). In addition, in vivo experiments have shown that the composite sponge promotes epithelial regeneration and collagen deposition in wounds infected with S. aureus or P. aeruginosa. Tissue immunofluorescence staining analysis confirmed that the SC/Se-Ag/Cur complex sponge stimulated upregulated expression of CD31 to promote angiogenesis while downregulating TNF-α expression to reduce inflammation. These advantages make it an ideal candidate for infectious wound repair materials, providing an effective repair strategy for clinical skin trauma infections.
理想的创伤敷料应具有优异的抗菌性能，并为再生受损皮肤组织提供适宜的微环境。在本研究中，我们利用丝胶原位合成银纳米粒子，并引入姜黄素，得到丝胶-银纳米粒子/姜黄素（Se-Ag/Cur）抗菌剂。然后，将该混合抗菌剂封装在物理双重交联的 3D 结构网络（海藻酸钠-壳聚糖，SC）中，得到 SC/Se-Ag/Cur 复合海绵。3D 结构网络通过海藻酸钠与壳聚糖之间的静电相互作用以及海藻酸钠与钙离子之间的离子相互作用构建。所制备的复合海绵具有优异的吸湿性（接触角 51.3°±5.6°）、保湿能力、孔隙率（67.32%±3.37%）和机械性能（>0.7 MPa），对铜绿假单胞菌（P. aeruginosa）和金黄色葡萄球菌（S. aureus）具有良好的抗菌能力。此外，体内实验表明，复合海绵促进了金黄色葡萄球菌或铜绿假单胞菌感染伤口的上皮再生和胶原沉积。组织免疫荧光染色分析证实，SC/Se-Ag/Cur 复合海绵刺激 CD31 的上调表达，促进血管生成，同时下调 TNF-α 的表达，减少炎症反应。这些优势使其成为感染性创伤修复材料的理想候选物，为临床皮肤创伤感染提供了有效的修复策略。
