Preparation and Biological Evaluation of Porous Tantalum Scaffolds Coated with Hydroxyapatite.

Orthopedic implants, such as porous scaffolds, are an effective way to repair bone defects. However, the lack of osseointegration and osteoinduction limits the achievement of an ideal therapeutic effect. This study aimed to prepare hydroxyapatite (HA) coatings for the surface of porous tantalum (Ta) scaffolds and to assess the effectively improved biological activities of the coated scaffolds. The porous Ta scaffolds were prepared by chemical vapor deposition, and then the porous Ta scaffolds were coated with HA via electrochemical deposition. The elements and phase compositions of the coatings were analyzed by energy-dispersive X-ray spectroscopy, X-ray diffraction, and X-ray photoelectron spectroscopy. The results showed that the coating covered the whole surfaces of porous Ta scaffolds with a uniform and compact distribution and did not exert any obvious effect on the porous structure. The biological activity of porous Ta scaffolds after surface modification increased and the water contact angle decreased, indicating that hydrophilicity was significantly improved. Cell live/dead staining, cytoskeletal fluorescence staining, and alkaline phosphatase immunofluorescence staining showed that the coating exhibited no cytotoxicity and notably improved cell proliferation, spreading, and osteogenic differentiation. In addition, in vivo experiments in animals have demonstrated that HA-coated porous Ta scaffolds contribute to bone formation. In conclusion, the HA coating notably improves the biological activities of the porous Ta scaffolds, achieving the goal of the present study. The HA coating presents great potential for the modification of porous Ta implants to improve their osteogenesis and osseointegration.