Borah Munmi, Mandal Manabendra, Konwar Bolin K
Department of Molecular Biology and Biotechnology, Tezpur University, Napaam, Tezpur-784028, Assam, India.
Int Microbiol. 2024 Sep 18. doi: 10.1007/s10123-024-00589-5.
The main objective of this study was to assess cellulolytic probiotic strains from traditional fermented beverages such as palm wine in order to supplement the animal feed and strengthen the gut health of the animal for better digestibility and absorption. In the present study, different types of microbes were isolated from traditionally prepared palm wine and analyzed for their probiotic nature. For any microbe to be probiotic in nature, it has to sustain the harsh conditions of the human gastrointestinal tract such as acid tolerance, bile tolerance at the lower range of pH, and other properties like auto aggregation test, cell surface hydrophobicity test with non-polar hydrocarbons for evaluating its capabilities to adhere to the intestinal cells and antimicrobial nature against pathogens. Bacillus mycoides strain PR04 and Bacillus subtilis strain PR21 were found to be resistant to acid and bile in simulated artificial gastrointestinal tract model, found to be than 55% hydrophobic with xylene and n-hexadecane and also showed antimicrobial activity greater towards pathogenic strains like Pseudomonas aeruginosa, Staphylococcus aureus, Candida albicans, and Salmonella typhimurium respectively. The cellulolytic activity of the isolates PR04 and PR21 was evaluated in (0.2-2) % CMC (carboxymethyl cellulose) plate. Bacillus mycoides PR04 and Bacillus subtilis PR21 could degrade carboxymethyl cellulose, filter paper, and sugarcane bagasse. The degradation of sugarcane bagasse was confirmed by Scanning electron microscopy and filter paper degradation after 4 days of incubation at 37 °C. Cellulase gene of the identified Bacillus sp. strains was amplified by primers CF5'-ACAGGATCCGATGAAAACGGTCAATTTCTATTTT-3' and CR5'-ACTCTCGAGATTGGGTTCTGTTCCCAAT-3'. This study proposes potential probiotic Bacillus mycoides PR04 (Accession no. OR625070) and Bacillus subtilis PR21 (Accession no. OR625072) in the application as an animal feed additive to assist in its digestibility and encourage the gut health.
本研究的主要目的是评估从传统发酵饮料如棕榈酒中分离出的纤维素分解益生菌菌株，以补充动物饲料并增强动物肠道健康，促进更好的消化和吸收。在本研究中，从传统制备的棕榈酒中分离出不同类型的微生物，并分析其益生菌特性。任何具有益生菌性质的微生物都必须能够在人类胃肠道的恶劣条件下生存，如耐酸性、在较低pH值范围内的耐胆汁性，以及其他特性，如自聚集试验、用非极性碳氢化合物进行细胞表面疏水性试验，以评估其粘附于肠道细胞的能力和对病原体的抗菌特性。在模拟人工胃肠道模型中发现，蕈状芽孢杆菌菌株PR04和枯草芽孢杆菌菌株PR21对酸和胆汁具有抗性，对二甲苯和正十六烷的疏水性超过55%，并且对铜绿假单胞菌、金黄色葡萄球菌、白色念珠菌和鼠伤寒沙门氏菌等致病菌株分别表现出更强的抗菌活性。在（0.2 - 2）%的羧甲基纤维素（CMC）平板上评估分离株PR04和PR21的纤维素分解活性。蕈状芽孢杆菌PR04和枯草芽孢杆菌PR21能够降解羧甲基纤维素、滤纸和甘蔗渣。在37℃孵育4天后，通过扫描电子显微镜确认了甘蔗渣的降解以及滤纸的降解。用引物CF5'-ACAGGATCCGATGAAAACGGTCAATTTCTATTTT-3'和CR5'-ACTCTCGAGATTGGGTTCTGTTCCCAAT-3'扩增已鉴定的芽孢杆菌属菌株的纤维素酶基因。本研究提出将潜在的益生菌蕈状芽孢杆菌PR04（登录号OR625070）和枯草芽孢杆菌PR21（登录号OR625072）用作动物饲料添加剂，以辅助其消化并促进肠道健康。
