COVID-19 患者支气管肺泡灌洗液中通过下一代测序鉴定的真菌：一项回顾性研究。
Fungi identified via next-generation sequencing in bronchoalveolar lavage fluid among patients with COVID-19: a retrospective study.
机构信息
Department of Critical Care Medicine, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
出版信息
Eur J Med Res. 2024 Sep 17;29(1):463. doi: 10.1186/s40001-024-02054-5.
BACKGROUND
The epidemiology of fungi identified via next-generation sequencing in bronchoalveolar lavage fluid among patients with COVID-19 is unknown.
METHODS
De-identified information, including age, SARS-CoV-2 reads and fungi from bronchoalveolar lavage fluid, were used to analysis.
RESULTS
A total of 960 patients with COVID-19 were included. Gender was unknown in 38 patients, and 648 (70.3%) of the rest patients were male. For 876 patients with information on age, their mean ± standard age was 63.4 ± 21.3 years, with the minimum being 0.2 years and the maximum being 101 years. For all the patients, their median [interquartile range] SARS-CoV-2 reads were 26,038 [4421.5, 44,641.5]. The Aspergilli were identified in 159 (16.6%) patients, with Aspergillus fumigatus, Aspergillus flavus and Aspergillus niger in 103 (10.7%), 81 (8.4%) and 17 (1.8%), respectively. The Mucoraceae were identified in 14 (1.5%) patients. Pneumocystis jirovecii was identified in 65 (6.8%) patients, among whom 12 (18.5%) patients also had Aspergilli. The Cryptococcaceae and the Dematiaceae were also identified in some patients, including Cryptococcus in 11 (1.1%) patients.
CONCLUSIONS
In bronchoalveolar lavage fluid among patients with COVID-19, the Aspergilli were very commonly identified, as were the Mucoraceae, Pneumocystis jirovecii and Cryptococcus via next-generation sequencing.
背景
COVID-19 患者支气管肺泡灌洗液中通过下一代测序鉴定的真菌的流行病学尚不清楚。
方法
使用鉴定信息，包括年龄、SARS-CoV-2 读取和支气管肺泡灌洗液中的真菌进行分析。
结果
共纳入 960 例 COVID-19 患者。38 例患者性别不详，其余 648 例（70.3%）为男性。对于 876 例有年龄信息的患者，其平均年龄为 63.4 ± 21.3 岁，年龄最小为 0.2 岁，最大为 101 岁。对于所有患者，其中位数[四分位数范围]SARS-CoV-2 读数为 26038[4421.5, 44641.5]。159 例（16.6%）患者中鉴定出曲霉菌，103 例（10.7%）、81 例（8.4%）和 17 例（1.8%）分别为烟曲霉、黄曲霉和黑曲霉。14 例（1.5%）患者中鉴定出毛霉科。65 例（6.8%）患者中鉴定出卡氏肺孢子菌，其中 12 例（18.5%）患者也有曲霉菌。一些患者中还鉴定出隐球菌科和暗色孢科，包括 11 例（1.1%）患者的隐球菌。
结论
在 COVID-19 患者的支气管肺泡灌洗液中，通过下一代测序非常常见地鉴定出曲霉菌、毛霉科、卡氏肺孢子菌和隐球菌。
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