Some interleukin-3 dependent mast-cell lines also respond to interleukin-2.

The haemopoietic stem cell line 32D cl-23 is alcian blue positive, Lyt 1+, and requires a growth factor, interleukin-3 (IL-3). This cell has now been shown to respond to interleukin-2 (IL-2). Antibodies directed against the murine interleukin-2 receptor blocked 3H-thymidine incorporation by 32D cl-23 cells grown in interleukin-2, but had no effect on 32D cl-23 in interleukin-3. Thus the receptor for interleukin-2 on 32D cl-23 cells may be similar to the interleukin-2 receptor on murine T cells. Similar results were obtained using a different basophil cell line 32D cl-5, but not using another basophil cell line, Rotundi, or factor dependent musocal type mast cells (P cells) derived from spleen.