Koyasu S, Yodoi J, Nikaido T, Tagaya Y, Taniguchi Y, Honjo T, Yahara I
J Immunol. 1986 Feb 1;136(3):984-7.
Several mouse IL 3-dependent cell lines, IC2, LT4, FDC-P2, and PB-3C, derived from spleen or bone marrow cells were shown to express low affinity receptors for IL 2 (Kd; 0.5 to 8 X 10(-8) M). High affinity receptors for IL 2 were not detected on the IL 3-dependent cells within the experimental limitation of this study. The clones did not respond to IL 2 at all at the concentration as high as 25 micrograms/ml. The number of the receptors expressed on those clones was estimated to be 0.2 to 2 X 10(5)/cell, which is comparable with the number of those on IL 2-dependent T cell clones. Expression of IL 2 receptor was confirmed in mRNA levels for both IC2 and LT4 cells. A relatively low level expression of one (4.5 Kb) of four IL 2 receptor mRNA species was observed with those IL 3-dependent clones compared with IL 2-dependent T cells. It seems that these low affinity receptors may be expressed on IL 3-dependent cells that undergo differentiation or maturation in mast cell and some myeloid cell lineages.
从脾脏或骨髓细胞中获得的几种小鼠白细胞介素3（IL 3）依赖细胞系，IC2、LT4、FDC-P2和PB-3C，被证明表达白细胞介素2（IL 2）的低亲和力受体（解离常数Kd；0.5至8×10⁻⁸M）。在本研究的实验限度内，未在IL 3依赖细胞上检测到IL 2的高亲和力受体。这些克隆在高达25微克/毫升的浓度下对IL 2完全没有反应。这些克隆上表达的受体数量估计为0.2至2×10⁵/细胞，这与IL 2依赖的T细胞克隆上的受体数量相当。在IC2和LT4细胞的mRNA水平上均证实了IL 2受体的表达。与IL 2依赖的T细胞相比，在这些IL 3依赖克隆中观察到四种IL 2受体mRNA种类之一（4.5千碱基）的表达水平相对较低。似乎这些低亲和力受体可能在肥大细胞和一些髓系细胞谱系中经历分化或成熟的IL 3依赖细胞上表达。
