Röseler Waltraud, Sommer Ralf J, Rödelsperger Christian
Department for Integrative Evolutionary Biology, Max Planck Institute for Biology, Max-Planck-Ring 9, 72076 Tübingen, Germany.
J Nematol. 2024 Sep 11;56(1):20240063. doi: 10.2478/jofnem-2024-0063. eCollection 2024 Mar.
is a free-living nematode that shares many features with , such as its short generation time and hermaphroditism, but also exhibits novel traits, i.e., a mouth-form dimorphism that enables predation. The availability of various genetic tools and genomic resources make it a powerful model organism for comparative studies. Here, we present an updated genome of the strain PS1843 (Washington) that is most widely used for genetic analysis. Assembly of PacBio reads together with reference-guided scaffolding resulted in a chromosome-scale genome spanning 171Mb for the PS1843 strain. Whole genome alignments between the PS1843 genome and the genome of the reference strain PS312 (California) revealed megabase-sized regions on chromosomes III, IV, and X that explain the majority of genome size difference between both strains. The improved PS1843 genome will be useful for future forward genetic studies and evolutionary genomic comparisons at the intra-species level.
是一种自由生活的线虫，与[某种线虫名称未给出]具有许多共同特征，如短世代时间和雌雄同体，但也表现出新颖的特征，即一种能够捕食的口器形态二态性。各种遗传工具和基因组资源的可用性使其成为比较研究的强大模式生物。在这里，我们展示了最广泛用于遗传分析的[线虫名称未给出]菌株PS1843（华盛顿）的更新基因组。PacBio读数的组装以及参考引导的支架构建产生了PS1843菌株跨越171Mb的染色体规模基因组。PS1843基因组与参考菌株PS312（加利福尼亚）的基因组之间的全基因组比对揭示了III、IV和X染色体上兆碱基大小的区域，这些区域解释了两个菌株之间基因组大小差异的大部分。改进后的PS1843基因组将有助于未来在物种内水平进行正向遗传学研究和进化基因组比较。
