Xia Simin, Chen Yue, Lai Jianbin, Zhang Zhonghui, Yang Chengwei, Han Danlu
Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, School of Life Science, South China Normal University, Guangzhou, China.
Plant Genome. 2024 Dec;17(4):e20511. doi: 10.1002/tpg2.20511. Epub 2024 Sep 18.
MicroRNAs (miRNAs) play an essential role as non-coding-RNA-type epigenetic regulators in response to high-temperature stress in plants. There are crucial roles for global transcriptional regulation under SUMO (small ubiquitin-related MOdifier) stress response (SSR). However, the molecular mechanisms underlying its downstream regulation remain unclear. In this study, SUMO-specific chromatin immunoprecipitation sequencing analysis detected specific binding in the promoter region of miRNAs under high-temperature stress. A correlation analysis between this binding and miRNA profiling revealed that the location of SUMO on the chromosome was correlated with the expression pattern of miRNAs, particularly miR398a and miR824a. In contrast, knockout mutants of the SSR-dependent SUMO E3 ligase SAP AND MIZ 1 in Arabidopsis exhibited opposing trends in target gene expression for the SUMO-related miRNAs compared to the wild type. Multi-omics correlation analyses identified 34 SUMO-candidate proteins that might be involved in the regulation of miRNA response to high-temperature stress. Therefore, we propose a potential model whereby high-temperature exposure induces nuclear entry of SUMO molecules, modifying specific transcription factors that bind to miRNA gene promoters and potentially regulate miRNA expression.
微小RNA（miRNA）作为非编码RNA类型的表观遗传调控因子，在植物应对高温胁迫中发挥着重要作用。在小泛素相关修饰物（SUMO）应激反应（SSR）下，全局转录调控起着关键作用。然而，其下游调控的分子机制仍不清楚。在本研究中，SUMO特异性染色质免疫沉淀测序分析检测到高温胁迫下miRNA启动子区域的特异性结合。这种结合与miRNA谱之间的相关性分析表明，SUMO在染色体上的位置与miRNA的表达模式相关，尤其是miR398a和miR824a。相反，拟南芥中依赖SSR的SUMO E3连接酶SAP AND MIZ 1的敲除突变体与野生型相比，SUMO相关miRNA的靶基因表达呈现相反趋势。多组学相关性分析确定了34种可能参与调控miRNA对高温胁迫反应的SUMO候选蛋白。因此，我们提出了一个潜在模型，即高温暴露诱导SUMO分子进入细胞核，修饰与miRNA基因启动子结合的特定转录因子，并可能调控miRNA表达。
