College of Food Science and Technology, Northwest University, Xi'an, Shaanxi, 710069, China.
Laboratory of Nutritional and Healthy Food-Individuation Manufacturing Engineering, Xi'an, Shaanxi, 710069, China.
Food Funct. 2024 Sep 30;15(19):10067-10087. doi: 10.1039/d4fo02256d.
Acute alcoholic liver injury (AALI) is a widespread disease that can develop into hepatitis, liver fibrosis, and cirrhosis. In severe cases, it can be life-threatening, while drug treatment presents various side effects. This study characterized the structure of natural lentinan (LNT) from the Qinba Mountain area and investigated the protective mechanism of different LNT doses (100 mg kg, 200 mg kg, and 400 mg kg) on AALI. The results showed that LNT was a glucose-dominated pyran polysaccharide with a triple-helical structure and a molecular weight () of 7.56 × 10 Da. An AALI mouse model showed that all the LNT doses protected liver function, reduced hepatic steatosis, alleviated oxidative stress and inflammatory response, and stimulated probiotic proliferation. Low-dose LNT increased anti-oxidant-associated beneficial bacteria, medium-dose LNT improved liver swelling and promoted anti-oxidant-associated probiotics, and high-dose LNT increased the probiotics that helped protect liver function and anti-oxidant and anti-inflammatory properties. All the LNT doses inhibited pathogenic growth, including , , , , , and . These results indicated that the protective effect of LNT against AALI was mediated by the proliferation of various intestinal probiotics and was related to the consumption doses. These findings offer new strategies for comprehensively utilizing from the Qinba Mountain area and preventing AALI using natural food-based substances.
急性酒精性肝损伤（AALI）是一种广泛存在的疾病，可发展为肝炎、肝纤维化和肝硬化。在严重的情况下，它可能危及生命，而药物治疗则存在各种副作用。本研究对来自秦巴山区的天然香菇多糖（LNT）的结构进行了表征，并研究了不同剂量（100mg/kg、200mg/kg 和 400mg/kg）的 LNT 对 AALI 的保护机制。结果表明，LNT 是一种以葡萄糖为主的吡喃多糖，具有三螺旋结构，分子量（）为 7.56×10 Da。AALI 小鼠模型表明，所有 LNT 剂量均能保护肝功能，减少肝脂肪变性，减轻氧化应激和炎症反应，刺激益生菌增殖。低剂量 LNT 增加了与抗氧化相关的有益细菌，中剂量 LNT 改善了肝脏肿胀并促进了与抗氧化相关的益生菌，高剂量 LNT 增加了有助于保护肝功能和抗氧化及抗炎特性的益生菌。所有 LNT 剂量均抑制了致病菌的生长，包括 、 、 、 、 和 。这些结果表明，LNT 对 AALI 的保护作用是通过各种肠道益生菌的增殖介导的，与消耗剂量有关。这些发现为综合利用秦巴山区的 并利用天然食物来源的物质预防 AALI 提供了新策略。
