Mersin University, Medical Faculty, Department of Medical Biochemistry - Mersin, Turkey.
Mersin University, Medical Faculty, Department of Rheumatology - Mersin, Turkey.
Rev Assoc Med Bras (1992). 2024 Sep 16;70(9):e20231724. doi: 10.1590/1806-9282.20231724. eCollection 2024.
Osteoporosis, defined as a systemic skeletal disease, is characterized by increased bone fragility and fracture risk. Studies have shown that dysregulation of the functions of miRNAs or the mechanisms they mediate may be an important pathological factor in bone degeneration. Therefore, the aim of the study was to determine the role of miRNAs, which are thought to play a role in bone metabolism, in osteoporosis.
The study included 48 patients who were diagnosed with osteoporosis according to the results of a bone mineral density assessment by quantitative computed tomography and 36 healthy individuals. MiRNAs from plasma samples obtained from blood samples taken into ethylenediaminetetraacetic acid (EDTA) tubes were isolated with the miRNA isolation kit and converted to cDNA. Expression analysis of miR-21-5p, miR-34a-5p, miR-210, miR-122-5p, miR-125b-5p, miR-133a, miR-143-3p, miR-146a, miR-155-5p, and miR-223 was performed on the real-time PCR (RT-PCR) device.
When miRNA expression levels in the patient group were compared with the control group, all miRNAs were found to be downregulated in the patients. When fold changes in expression levels in the patient group were examined, significant differences were found in miR-21-5p, miR-133a, mir143-3p, miR-210, and miR-223. In the receiver operating curve analysis, area under the curve=0.882 for the combination of miR-34, miR-125, miR-133, and miR-210.
In this study, it was determined that the combined effects of miRNAs, as well as their single effects, were effective in the development of osteoporosis. Therefore, a miRNA panel to be created can make a significant contribution to the development of novel diagnostic and treatment approaches for this disease.
骨质疏松症是一种全身性骨骼疾病，其特征是骨脆性增加和骨折风险增加。研究表明，miRNA 功能的失调或它们介导的机制可能是骨骼退化的一个重要病理因素。因此，本研究旨在确定被认为在骨代谢中起作用的 miRNAs 在骨质疏松症中的作用。
该研究纳入了 48 名根据定量计算机断层扫描骨矿物质密度评估结果诊断为骨质疏松症的患者和 36 名健康个体。使用 miRNA 分离试剂盒从 EDTA 管采集的血浆样本中分离 miRNA 并转化为 cDNA。使用实时 PCR（RT-PCR）设备对 miR-21-5p、miR-34a-5p、miR-210、miR-122-5p、miR-125b-5p、miR-133a、miR-143-3p、miR-146a、miR-155-5p 和 miR-223 的表达进行分析。
与对照组相比，患者组的所有 miRNA 表达水平均下调。当检查患者组表达水平的倍数变化时，miR-21-5p、miR-133a、mir143-3p、miR-210 和 miR-223 有显著差异。在受试者工作特征曲线分析中，miR-34、miR-125、miR-133 和 miR-210 的组合曲线下面积为 0.882。
在这项研究中，确定了 miRNA 的联合作用及其单一作用在骨质疏松症的发展中是有效的。因此，创建一个 miRNA 面板可以为该疾病的新诊断和治疗方法的发展做出重大贡献。
