Department of Health and Exercise Science, University of Oklahoma, 1401 Asp Avenue, Norman, OK 73019, USA.
Department of Health and Exercise Science, University of Oklahoma, 1401 Asp Avenue, Norman, OK 73019, USA.
Bone. 2019 Mar;120:271-278. doi: 10.1016/j.bone.2018.11.001. Epub 2018 Nov 5.
MicroRNAs (miRNAs) are short, non-coding RNA molecules that fine tune posttranscriptional protein expression. Aging is accompanied by progressive declines in muscle mass and strength, and in bone mineral density (BMD). Although miRNAs in pathology have been extensively studied, the role of circulating miRNAs (c-miRNAs) in osteoporosis and sarcopenia has to date not been well understood. The purpose of this study was to examine the difference in bone and muscle specific c-miRNAs in postmenopausal women based on their bone and muscle status, and to determine the associations between these specific c-miRNAs and muscle and bone variables. Seventy-five postmenopausal women aged 60 to 85 years old participated in this study. Body composition and BMD, functional performance tests (grip strength, gait speed, and countermovement jumps) were assessed. Levels of c-miRNAs (miR-1-3p, -21-5p, -23a-3p, -24-3p, -100-5p, -125b-5p, -133a-3p, -206) and bone turnover markers were analyzed. Statistically, there were no significant differences in specific c-miRNAs based on sarcopenia and osteoporosis status. However, fold changes of miR-21-5p (FC = 2.59) and -23a-3p (FC = 2.09) indicated upregulation and miR-125b-5p (FC = 0.46) indicated downregulation in the osteoporotic group compared to the non-osteoporotic group. The relative expression level of miR-125b-5p was significantly positively correlated with age (p < 0.05). The relative expression level of miR-21-5p was significantly negatively correlated with trochanter BMC (p < 0.05). Furthermore, the relative expression level of miR-23a-3p was significantly positively correlated with TRAP5b levels (p < 0.05). Although no statistical differences were found in target c-miRNAs based on muscle and bone status, our results indicate that there are biological differential expressions in some c-miRNAs between osteoporotic and non-osteoporotic individuals. Other circulating miRNAs need to be studied in the future.
微小 RNA（miRNA）是一类短链、非编码 RNA 分子，可精细调控转录后蛋白的表达。衰老伴随着肌肉量和力量以及骨矿物质密度（BMD）的逐渐下降。尽管病理 miRNA 已得到广泛研究，但循环 miRNA（c-miRNA）在骨质疏松症和肌肉减少症中的作用尚未得到充分理解。本研究旨在根据绝经后妇女的骨骼和肌肉状况，检测骨和肌肉特异性 c-miRNA 的差异，并确定这些特异性 c-miRNA 与肌肉和骨骼变量之间的关联。本研究纳入了 75 名年龄在 60 至 85 岁之间的绝经后妇女。评估了身体成分和 BMD、功能性能测试（握力、步态速度和反向跳跃）。分析了 c-miRNA（miR-1-3p、-21-5p、-23a-3p、-24-3p、-100-5p、-125b-5p、-133a-3p、-206）和骨转换标志物的水平。统计分析显示，基于肌肉减少症和骨质疏松症的状态，c-miRNA 的特异性没有显著差异。然而，miR-21-5p（FC=2.59）和 -23a-3p（FC=2.09）的倍数变化表明，与非骨质疏松组相比，骨质疏松组的表达上调，而 miR-125b-5p（FC=0.46）的表达下调。miR-125b-5p 的相对表达水平与年龄呈显著正相关（p<0.05）。miR-21-5p 的相对表达水平与转子间骨矿物质密度呈显著负相关（p<0.05）。此外，miR-23a-3p 的相对表达水平与 TRAP5b 水平呈显著正相关（p<0.05）。尽管基于肌肉和骨骼状况，靶 c-miRNA 没有统计学差异，但我们的结果表明，在一些 c-miRNA 中，骨质疏松症和非骨质疏松症个体之间存在生物学差异表达。未来需要研究其他循环 miRNA。
