通过亲和色谱法纯化细胞分裂素N6-（δ2-异戊烯基）腺苷的抗体。
Purification of antibodies for the cytokinin N6-(delta 2-isopentenyl) adenosine by affinity chromatography.
作者信息
Senapathy P, Jayabaskaran C, Jacob M T
出版信息
Nucleic Acids Res. 1985 Aug 26;13(16):5833-42. doi: 10.1093/nar/13.16.5833.
Abstract
Isopentenyl adenosine antibodies useful in the investigations of the "cytokinin" functions of isopentenyl adenosine were purified by affinity chromatography. Using different affinity columns, the antibodies were purified to near complete purity. Analyses of the purified proteins revealed the presence of isopentenyl adenosine binding proteins in normal rabbit serum, which presence supports a suggested role for isopentenyl adenosine and its related compounds in animal cell division in vivo.
摘要
用于研究异戊烯基腺苷“细胞分裂素”功能的异戊烯基腺苷抗体通过亲和层析法进行纯化。使用不同的亲和柱，抗体被纯化至近乎完全的纯度。对纯化蛋白质的分析揭示了正常兔血清中存在异戊烯基腺苷结合蛋白，这一存在支持了异戊烯基腺苷及其相关化合物在体内动物细胞分裂中的假定作用。
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