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通过亲和色谱法纯化细胞分裂素N6-（δ2-异戊烯基）腺苷的抗体。

Purification of antibodies for the cytokinin N6-(delta 2-isopentenyl) adenosine by affinity chromatography.

作者信息

Senapathy P, Jayabaskaran C, Jacob M T

出版信息

Nucleic Acids Res. 1985 Aug 26;13(16):5833-42. doi: 10.1093/nar/13.16.5833.

DOI:10.1093/nar/13.16.5833
PMID:3929232
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC321915/
Abstract

Isopentenyl adenosine antibodies useful in the investigations of the "cytokinin" functions of isopentenyl adenosine were purified by affinity chromatography. Using different affinity columns, the antibodies were purified to near complete purity. Analyses of the purified proteins revealed the presence of isopentenyl adenosine binding proteins in normal rabbit serum, which presence supports a suggested role for isopentenyl adenosine and its related compounds in animal cell division in vivo.

摘要

用于研究异戊烯基腺苷“细胞分裂素”功能的异戊烯基腺苷抗体通过亲和层析法进行纯化。使用不同的亲和柱，抗体被纯化至近乎完全的纯度。对纯化蛋白质的分析揭示了正常兔血清中存在异戊烯基腺苷结合蛋白，这一存在支持了异戊烯基腺苷及其相关化合物在体内动物细胞分裂中的假定作用。

相似文献

1
Purification of antibodies for the cytokinin N6-(delta 2-isopentenyl) adenosine by affinity chromatography.通过亲和色谱法纯化细胞分裂素N6-（δ2-异戊烯基）腺苷的抗体。
Nucleic Acids Res. 1985 Aug 26;13(16):5833-42. doi: 10.1093/nar/13.16.5833.
2
Identification and purification of tRNAs containing N6-(delta 2-isopentenyl) adenosine using antibodies specific for N6-(delta-isopentenyl) adenosine.使用对N6-（δ-异戊烯基）腺苷具有特异性的抗体鉴定和纯化含有N6-（δ2-异戊烯基）腺苷的tRNA。
J Biol Chem. 1981 Nov 25;256(22):11580-4.
3
Immunospecific binding of tRNA precursors containing N6-(delta 2-isopentenyl) adenosine.含有N6-（δ2-异戊烯基）腺苷的tRNA前体的免疫特异性结合。
Biochem Int. 1984 Feb;8(2):257-64.
4
New synthesis of immunogenic N6-isopent-2-enyladenosine-protein conjugate. Preparation, purification, and specificity of related antibodies.免疫原性N6-异戊-2-烯基腺苷-蛋白质缀合物的新合成。相关抗体的制备、纯化及特异性。
Bioconjug Chem. 1992 Jan-Feb;3(1):14-9. doi: 10.1021/bc00013a002.
5
A rapid and sensitive auxin binding system for detecting N6-substituted adenines, and some urea and thiourea derivatives, that show cytokinin activity in cell division tests.一种快速灵敏的生长素结合系统，用于检测在细胞分裂试验中显示细胞分裂素活性的N6-取代腺嘌呤以及一些尿素和硫脲衍生物。
Can J Biochem. 1975 Jul;53(7):768-78. doi: 10.1139/o75-104.
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Presence and source of free isopentenyladenosine in yeasts.酵母中游离异戊烯基腺苷的存在及来源
Proc Natl Acad Sci U S A. 1985 Feb;82(4):1113-5. doi: 10.1073/pnas.82.4.1113.
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Purification and specificity of antibodies to adenosine.腺苷抗体的纯化及特异性
Biochimie. 1977;59(1):33-42. doi: 10.1016/s0300-9084(77)80083-7.
8
Antibodies to N6-(delta2-isopentenyl) adenosine and its nucleotide: interaction with purified tRNAs and with bases, nucleosides and nucleotides of the isopentenyladenosine family.抗N6-（δ2-异戊烯基）腺苷及其核苷酸的抗体：与纯化的tRNA以及异戊烯基腺苷家族的碱基、核苷和核苷酸的相互作用。
Nucleic Acids Res. 1978 Sep;5(9):3439-55. doi: 10.1093/nar/5.9.3439.
9
Cytokinin oxidase activity and cytokinin content in roots of sunflower under water stress.水分胁迫下向日葵根系中细胞分裂素氧化酶活性与细胞分裂素含量
Indian J Exp Biol. 2001 Aug;39(8):786-92.
10
Cytokinins: Metabolism and Biological Activity of N-(Delta-Isopentenyl)adenosine and N-(Delta-Isopentenyl)adenine in Tobacco Cells and Callus.细胞分裂素：烟草细胞和愈伤组织中N-（Δ-异戊烯基）腺苷和N-（Δ-异戊烯基）腺嘌呤的代谢与生物活性
Plant Physiol. 1977 Mar;59(3):478-83. doi: 10.1104/pp.59.3.478.

本文引用的文献

1
A Cytokinin (Isopentenyl-Adenosyl-Mononucleotide) Linked to Ecdysone in Newly Laid Eggs of Locusta migratoria.蜕皮激素在新产卵中的细胞分裂素（异戊烯腺嘌呤核苷酸）的联系。
Science. 1983 Apr 29;220(4596):507-9. doi: 10.1126/science.220.4596.507.
2
ANTIBODIES SPECIFIC FOR RIBONUCLEOSIDES AND RIBONUCLEOTIDES AND THEIR REACTION WITH DNA.对核糖核苷和核糖核苷酸具有特异性的抗体及其与DNA的反应。
Proc Natl Acad Sci U S A. 1964 Jul;52(1):68-74. doi: 10.1073/pnas.52.1.68.
3
Immunologic studies on nucleic acids and their components. II. Reversible inhibition of anti-nucleoside antibodies in aqueous pyridine and its application in antibody purification.
核酸及其组分的免疫学研究。II. 吡啶水溶液中抗核苷抗体的可逆抑制及其在抗体纯化中的应用。
Biochim Biophys Acta. 1974 Apr 27;349(1):84-95. doi: 10.1016/0005-2787(74)90011-2.
4
Identification and purification of tRNAs containing N6-(delta 2-isopentenyl) adenosine using antibodies specific for N6-(delta-isopentenyl) adenosine.使用对N6-（δ-异戊烯基）腺苷具有特异性的抗体鉴定和纯化含有N6-（δ2-异戊烯基）腺苷的tRNA。
J Biol Chem. 1981 Nov 25;256(22):11580-4.
5
Magnesium-dependent interaction of 30S ribosomal subunits with antibodies to N6, N6-dimethyladenosine.30S核糖体亚基与N6,N6-二甲基腺苷抗体的镁依赖性相互作用
Biochemistry. 1980 Aug 5;19(16):3786-91. doi: 10.1021/bi00557a021.
6
Immunoelectron microscopic localization of the site of photo-induced affinity labeling of the small ribosomal subunit with puromycin.用嘌呤霉素对小核糖体亚基进行光诱导亲和标记位点的免疫电子显微镜定位。
Proc Natl Acad Sci U S A. 1980 Feb;77(2):890-4. doi: 10.1073/pnas.77.2.890.
7
Isopentenyladenosine stimulates and inhibits mitosis of human lymphocytes treated with phytohemagglutinin.异戊烯基腺苷刺激并抑制经植物血凝素处理的人淋巴细胞的有丝分裂。
Science. 1969 Jul 25;165(3891):400-2. doi: 10.1126/science.165.3891.400.
8
N6-(delta-2-isopentenyl) adenosine--effects upon nucleic acid synthesis in lymphocytes in vitro and the development of immunologic hypersensitivity in vivo.N6-（δ-2-异戊烯基）腺苷——对体外淋巴细胞核酸合成及体内免疫超敏反应发展的影响。
Biochem Pharmacol. 1969 Apr;18(4):847-53. doi: 10.1016/0006-2952(69)90055-0.
9
Toxicological and antiproliferative effects of N6-(delta2-isopentenyl) adenosine, a natural component of mammalian transfer RNA.N6-（δ2-异戊烯基）腺苷的毒理学和抗增殖作用，一种哺乳动物转移RNA的天然成分。
Cancer Res. 1970 May;30(5):1429-36.
10
Formation of an antibody with serologic specificity for N 6 -( 2 -isopentenyl) adenosine.具有针对N6-（2-异戊烯基）腺苷血清学特异性的抗体的形成。
J Immunol. 1972 Jun;108(6):1726-8.