The Institute of Natural and Applied Sciences, Biotechnology Department, Ege University, Bornova, Izmir 35100, Turkey; Department of Analytical Chemistry, Faculty of Pharmacy, Ege University, Bornova, Izmir 35100, Turkey.
Department of Analytical Chemistry, Faculty of Pharmacy, Ege University, Bornova, Izmir 35100, Turkey; Biomedical Device Technology Program, Vocational School of Health Services, Izmir Democracy University, Izmir 35140, Turkey.
J Biotechnol. 2024 Nov 20;395:64-70. doi: 10.1016/j.jbiotec.2024.09.007. Epub 2024 Sep 16.
In this study, a novel electrochemical biosensor was developed for the sensitive and selective detection of the Acinetobacter baumannii gene sequence. The biosensor was created by immobilizing a capture probe specific to the A. baumannii gene on the surface of chitosan-gold modified pencil graphite electrodes. Following solid-state hybridization on the Chit-Au/PGE surface, the target DNA sequence of the A. baumannii was detected by measuring the guanine signal using square wave voltammetry (SWV). All experimental parameters impacting sensor response are examined in order to improve hybridization efficacy, and the electrochemical biosensor's performance. The limit of detection (LOD) for the A. baumannii gene sequence was calculated and found to be 1.93 nM. Three different non-complementary DNA sequences were used to evaluate the assay selectivity, but no interference effect was obtained. Additionally, the potential applicability of the biosensor to real samples was tested in artificial serum media. The suggested electrochemical test procedure is simple, approachable, and quick, making it a convenient approach for the screening of DNA sequence.
在这项研究中，开发了一种新型电化学生物传感器，用于灵敏和选择性地检测鲍曼不动杆菌基因序列。该生物传感器通过将针对鲍曼不动杆菌基因的捕获探针固定在壳聚糖-金修饰的铅笔状石墨电极表面上而制成。在 Chit-Au/PGE 表面上进行固态杂交后，通过使用方波伏安法 (SWV) 测量鸟嘌呤信号来检测鲍曼不动杆菌的目标 DNA 序列。为了提高杂交效率和电化学生物传感器的性能，检查了影响传感器响应的所有实验参数。计算了鲍曼不动杆菌基因序列的检测限 (LOD)，发现为 1.93 nM。使用三种不同的非互补 DNA 序列来评估测定法的选择性，但未获得干扰效应。此外，还在人工血清介质中测试了生物传感器对实际样品的潜在适用性。该建议的电化学测试程序简单、可行且快速，是筛选 DNA 序列的便捷方法。
