Biomedical Device Technology Program, Vocational School of Health Services, Izmir Democracy University, Izmir 35140, Turkey; Department of Analytical Chemistry, Faculty of Pharmacy, Ege University, Izmir 35100, Turkey.
Talanta. 2022 Nov 1;249:123696. doi: 10.1016/j.talanta.2022.123696. Epub 2022 Jun 16.
A new genosensor, which allows sensitive and selective detection of Acinetobacter baumannii gene sequence was developed herein. In this assay, capture probe of Acinetobacter baumannii was immobilized on the surface of chitosan modified single-use pencil graphite electrodes (c-PGEs) to obtain Acinetobacter baumannii genosensor. Then, Acinetobacter baumannii target DNA sequence was recognized after solid-state hybridization on c-PGE genosensor by measuring guanine signal via differential pulse voltammetry (DPV). In order to improve hybridization efficiency, experimental parameters affecting all assay steps are studied and the analytical performance of the genosensor was tested. The low limit of detection (LOD) for Acinetobacter baumannii target DNA sequence was obtained as 1.86 nM with developed genosensor. The selectivity of the proposed assay was then tested in the presence of 1-base mismatch, or two different type of non-complementary sequences and no interference effect was observed. The proposed electrochemical assay protocol is easy, convenient, and rapid which can be a decent alternative to existing methods.
本文开发了一种新的基因传感器，可用于灵敏和选择性地检测鲍曼不动杆菌基因序列。在该测定法中，将鲍曼不动杆菌的捕获探针固定在壳聚糖修饰的一次性铅笔式石墨电极（c-PGE）的表面上，以获得鲍曼不动杆菌基因传感器。然后，通过差分脉冲伏安法（DPV）测量鸟嘌呤信号，在 c-PGE 基因传感器上通过固态杂交来识别鲍曼不动杆菌靶 DNA 序列。为了提高杂交效率，研究了影响所有测定步骤的实验参数，并测试了基因传感器的分析性能。用开发的基因传感器获得了鲍曼不动杆菌靶 DNA 序列的低检测限（LOD）为 1.86 nM。然后，在存在 1 个碱基错配或 2 种不同类型的非互补序列的情况下测试了该测定法的选择性，未观察到干扰作用。该电化学测定法方案简单、方便、快速，可作为现有方法的良好替代方法。
