An electrochemical assay for sensitive detection of Acinetobacter baumannii gene.

A new genosensor, which allows sensitive and selective detection of Acinetobacter baumannii gene sequence was developed herein. In this assay, capture probe of Acinetobacter baumannii was immobilized on the surface of chitosan modified single-use pencil graphite electrodes (c-PGEs) to obtain Acinetobacter baumannii genosensor. Then, Acinetobacter baumannii target DNA sequence was recognized after solid-state hybridization on c-PGE genosensor by measuring guanine signal via differential pulse voltammetry (DPV). In order to improve hybridization efficiency, experimental parameters affecting all assay steps are studied and the analytical performance of the genosensor was tested. The low limit of detection (LOD) for Acinetobacter baumannii target DNA sequence was obtained as 1.86 nM with developed genosensor. The selectivity of the proposed assay was then tested in the presence of 1-base mismatch, or two different type of non-complementary sequences and no interference effect was observed. The proposed electrochemical assay protocol is easy, convenient, and rapid which can be a decent alternative to existing methods.