Tianjin Hospital, Tianjin University, Tianjin 300211, People's Republic of China; Tianjin Key Laboratory of Orthopedic Biomechanics and Medical Engineering, Tianjin Hospital, Tianjin 300050, People's Republic of China.
Centre for Translational Medicine Research & Development, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, People's Republic of China.
Int J Biol Macromol. 2024 Nov;280(Pt 2):135721. doi: 10.1016/j.ijbiomac.2024.135721. Epub 2024 Sep 16.
The management of bone defects, particularly those with irregular geometries resulting from osteoporotic fractures, remains fraught with challenges. Microspheres have emerged as a promising vehicle for tissue engineering, distinguished by their controlled release, safety, and ease of application. Various bioactive components are integrated into microspheres to improve their performance. Metal-organic frameworks, formed from metal ions interconnected by organic ligands, are increasingly utilized in tissue engineering. Specifically, magnesium-based MOFs are notable for their broad applicability; Mg ions are instrumental in bone reconstruction and repair, facilitating osteogenesis, angiogenesis, antibacterial effects, and anti-inflammatory properties. Mg-MOF was synthesized using magnesium chloride and gallic acid, and it was incorporated into gelatin microspheres to create Gel@Mg-MOF composite microspheres. Leveraging gelatin's biocompatibility, controlled release, and biodegradability, the composites' biocompatibility was evaluated through toxicity and adhesion assays. Moreover, the osteogenic and angiogenic potentials of the Gel@Mg-MOF microspheres were assessed, alongside their capacity for ROS scavenging. Results suggest that controlled Mg release from Gel@Mg-MOF microspheres promotes osteogenic activity in RBMSCs and enhances angiogenic potential in HUVECs. Additionally, the gallic acid-containing composite microspheres exhibited antioxidative properties. Collectively, the findings suggest that Gel@Mg-MOF microspheres could provide effective support for bone defect repair, with potential for clinical deployment.
骨缺损的管理，特别是那些由骨质疏松性骨折引起的不规则几何形状的骨缺损，仍然充满挑战。微球已成为组织工程的一种有前途的载体，其特点是可控释放、安全性和易于应用。各种生物活性成分被整合到微球中以提高其性能。金属有机框架由金属离子通过有机配体相互连接而成，在组织工程中越来越多地被应用。具体来说，基于镁的 MOFs 因其广泛的适用性而引人注目；Mg 离子在骨骼重建和修复中起着重要作用，促进成骨、血管生成、抗菌作用和抗炎特性。使用氯化镁和没食子酸合成了 Mg-MOF，并将其掺入明胶微球中，以制备 Gel@Mg-MOF 复合微球。利用明胶的生物相容性、控制释放和可生物降解性，通过毒性和黏附实验评估了复合材料的生物相容性。此外，还评估了 Gel@Mg-MOF 微球的成骨和血管生成潜力，以及其清除 ROS 的能力。结果表明，Gel@Mg-MOF 微球中 Mg 的控制释放促进了 RBMSCs 的成骨活性，并增强了 HUVECs 的血管生成潜力。此外，含有没食子酸的复合微球表现出抗氧化特性。总的来说，这些发现表明 Gel@Mg-MOF 微球可为骨缺损修复提供有效的支持，具有临床应用的潜力。
