Key Laboratory of Phytochemistry and Natural Medicines, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201, China.
Kunming College of Life Science, University of Chinese Academy of Sciences, Kunming, 650201, China.
Sci Data. 2024 Sep 18;11(1):1012. doi: 10.1038/s41597-024-03861-5.
Coptis teeta Wall. (Ranunculaceae), an endangered plant species of significant medicinal value, predominantly undergoes clonal propagation, potentially compromising the species' evolutionary potential and ultimately increase its risk of extinction. In this study, we successfully assembled two sets of haploid genomes (Hap1 and Hap2) for C. teeta, comprising nine homologous chromosome pairs, by employing Illumina and PacBio sequencing technologies. The genome annotation identified a total of 43,979 and 46,311 protein-coding genes in Hap1 and in Hap2, and most of them were functionally annotated. The high-quality reference genome will serve as an indispensable genomic resource for conservation and comprehensive exploitation of this endangered species. Between the two haploid genomes, numerous structural alterations were detected within the nine homologous chromosome pairs, potentially resulting in aberrant synapsis and irregular chromosomal segregation and thus contributing to the sustained preservation of clonal propagation in C. teeta. The findings offer new perspective for elucidating the genetic mechanism underlying the compromised sexual reproductive capacity of C. teeta, thereby facilitating its enhancement though molecular breeding and genetic improvement.
黄连（毛茛科）是一种具有重要药用价值的濒危植物物种，主要通过无性繁殖进行繁殖，这可能会损害物种的进化潜力，并最终增加其灭绝的风险。在这项研究中，我们成功地通过 Illumina 和 PacBio 测序技术为黄连组装了两套单倍体基因组（Hap1 和 Hap2），包含九个同源染色体对。基因组注释共鉴定出 Hap1 和 Hap2 中分别有 43979 和 46311 个蛋白质编码基因，其中大多数基因具有功能注释。高质量的参考基因组将成为保护和综合开发这种濒危物种不可或缺的基因组资源。在这两个单倍体基因组之间，在九个同源染色体对中检测到许多结构改变，这可能导致异常的联会和不规则的染色体分离，从而有助于黄连持续的无性繁殖。这些发现为阐明黄连受损的有性生殖能力的遗传机制提供了新的视角，从而通过分子育种和遗传改良来增强其生殖能力。
