Zhang Rongxiang, Wnag Maohui, Zhang Guiyu, Gao Xiaoman, Xiang Niyan, Liu Shuwen, Zhao Yuemei, Qin Lijun, Yuan Tao
School of Biological Science, Guizhou Education University, Guiyang, 550018, China.
State Key Laboratory of Hybrid Rice, Laboratory of Plant Systematics and Evolutionary Biology, College of Life Sciences, Wuhan University, Wuhan, 430072, China.
Sci Data. 2025 Apr 30;12(1):716. doi: 10.1038/s41597-025-05025-5.
Rosa laevigata Michx., an endemic perennial herbaceous plant in China, possesses significant medicinal value in traditional herbal practices. However, the absence of a reference genome has hindered its development and utilization. In this study, we present a chromosome-level de novo genome assembly of two haplotypes (Hap1 and Hap2) of R. laevigata by integration of Hifi long reads, BGI short reads, and Hi-C reads. The assembled Hap1 genome spans 493 Mb, Hap2 genome spans 479 Mb, and both of them were assigned to 7 chromosomes each. The mapping rate of BGI short reads to the two haplotypes genome is approximately 99.26% and 99.23%, and BUSCO assessment reveals that 98.6% and 98.7% of the genes are complete. Furthermore, we predicted 43,480 and 41,251 protein-coding genes in two haplotype genomes, respectively. The chromosome-level genome of R. laevigata enhances the genetic resources available for Rosa species and lays the groundwork for subsequent medicinal development.
金樱子是中国特有的多年生草本植物，在传统草药实践中具有重要的药用价值。然而，缺乏参考基因组阻碍了其开发和利用。在本研究中，我们通过整合Hifi长读段、BGI短读段和Hi-C读段，对金樱子的两个单倍型（Hap1和Hap2）进行了染色体水平的从头基因组组装。组装后的Hap1基因组跨度为493 Mb，Hap2基因组跨度为479 Mb，两者均被分配到7条染色体上。BGI短读段对两个单倍型基因组的映射率分别约为99.26%和99.23%，BUSCO评估显示98.6%和98.7%的基因是完整的。此外，我们在两个单倍型基因组中分别预测了43,480和41,251个蛋白质编码基因。金樱子的染色体水平基因组增加了蔷薇属物种可用的遗传资源，并为后续的药用开发奠定了基础。
