Chromosomal level genome assembly of medicinal plant Rosa laevigata.

Rosa laevigata Michx., an endemic perennial herbaceous plant in China, possesses significant medicinal value in traditional herbal practices. However, the absence of a reference genome has hindered its development and utilization. In this study, we present a chromosome-level de novo genome assembly of two haplotypes (Hap1 and Hap2) of R. laevigata by integration of Hifi long reads, BGI short reads, and Hi-C reads. The assembled Hap1 genome spans 493 Mb, Hap2 genome spans 479 Mb, and both of them were assigned to 7 chromosomes each. The mapping rate of BGI short reads to the two haplotypes genome is approximately 99.26% and 99.23%, and BUSCO assessment reveals that 98.6% and 98.7% of the genes are complete. Furthermore, we predicted 43,480 and 41,251 protein-coding genes in two haplotype genomes, respectively. The chromosome-level genome of R. laevigata enhances the genetic resources available for Rosa species and lays the groundwork for subsequent medicinal development.