猪胸膜肺炎放线杆菌菌株的生化及血清学鉴定
Biochemical and serological identification of strains of Haemophilus pleuropneumoniae.
作者信息
Lombin L H, Rosendal S, DeMoor J
出版信息
Vet Microbiol. 1985 Jun;10(4):393-7. doi: 10.1016/0378-1135(85)90010-0.
Eighteen field isolates of Haemophilus pleuropneumoniae were studied biochemically and serotyped using the complement fixation test (CFT), agglutination test and the immunodiffusion test. Three biochemical tests (V-dependency, CAMP-reaction and urease activity) were found to be very useful for the biochemical characterization of the H. pleuropneumoniae. Haemolysis on blood agar plates, although present, was not sufficiently pronounced in all cases to warrant absolute dependence on this characteristic. Serological typing revealed the isolates belong to Serotypes 1 and 5. The immunodiffusion test proved to be the most serotype specific, while a marked cross-reaction was observed with the CFT.
对18株胸膜肺炎放线杆菌的野外分离株进行了生化研究，并使用补体结合试验（CFT）、凝集试验和免疫扩散试验进行血清分型。发现三项生化试验（V因子依赖性、CAMP反应和脲酶活性）对胸膜肺炎放线杆菌的生化特性鉴定非常有用。血琼脂平板上的溶血现象虽然存在，但在所有情况下都不够明显，不足以完全依赖这一特性。血清学分型显示这些分离株属于1型和5型。免疫扩散试验被证明是最具血清型特异性的，而补体结合试验则观察到明显的交叉反应。
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