Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base of Ministry of Science and Technology, Institute of Food Safety and Nutrition, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China.
School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China.
J Agric Food Chem. 2024 Oct 2;72(39):21650-21666. doi: 10.1021/acs.jafc.4c06376. Epub 2024 Sep 18.
The β-type anti-Id (Ab2β) is considered to have potential for simulating the structure and function of the antigen. In this study, a β-type anti-Id (3A7 anti-I-GEAb) of the Cry1C toxin was captured from a GEAb library. Subsequently, a higher activity of mutant (3A7 mutant 8) was obtained from the mutagenesis library based on 3A7 anti-I-GEAb. The LD values of 3A7 anti-I-GEAb and 3A7 mutant 8 reach up to 38.9% and 46.8% of Cry1C toxin for and reach up to 32.9% and 37.4% of Cry1C toxin for . Additionally, an IC-ELISA was established based on 3A7 mutant 8 (as the coated "antigen"), with an LOD value of 0.35 ng/mL, exhibiting good accuracy and stability for detecting Cry1C toxin in spiked samples. The present β-type anti-I-GEAb not only exhibits insecticidal activity similar to Cry1C toxin, offering potential for environmentally friendly pest management, but it can also replace the Cry1C toxin structure to establish a highly sensitive and specific IC-ELISA for monitoring Cry1C toxin.
β 型抗独特型抗体（Ab2β）被认为具有模拟抗原结构和功能的潜力。在这项研究中，从 GEAb 文库中捕获了 Cry1C 毒素的β 型抗独特型抗体（3A7 抗-I-GEAb）。随后，基于 3A7 抗-I-GEAb 从突变文库中获得了具有更高活性的突变体（3A7 突变体 8）。3A7 抗-I-GEAb 和 3A7 突变体 8 的 LD 值分别达到 Cry1C 毒素的 38.9%和 46.8%，对 的 LD 值分别达到 Cry1C 毒素的 32.9%和 37.4%。此外，基于 3A7 突变体 8（作为包被的“抗原”）建立了 IC-ELISA，其 LOD 值为 0.35 ng/mL，对添加 Cry1C 毒素的样品具有良好的准确性和稳定性。本研究中的β 型抗独特型抗体不仅表现出与 Cry1C 毒素相似的杀虫活性，为环保型害虫管理提供了潜力，而且还可以替代 Cry1C 毒素结构，建立高度敏感和特异的 IC-ELISA 来监测 Cry1C 毒素。
