Stanislavsky E S, Joo I, Mashilova G M, Boolk V F, Boltutsy L G, Zakgeim D A, Severtśova M K, Yedvabnaya L S, Gladus M A, Fatkhinurova T I
Vaccine. 1985 Jun;3(2):128-36. doi: 10.1016/0264-410x(85)90062-3.
An experimental study of several types of vaccines against Pseudomonas aeruginosa has been carried out. Pyoimmunogen, a multi-component vaccine consisting mainly of heat-stable proteins (or polypeptides) with a molecular weight of 20 000 to 60 000, was prepared both by laboratory and production techniques using three or five selected strains of P. aeruginosa. The preparations obtained by both methods were found to be of low toxicity and to induce in mice and rats active immunity against challenge with homologous and some heterologous strains of P. aeruginosa, including the toxigenic PA-103 strain. The Pseudomonas vaccine (PV) was prepared from partly purified water-soluble protein antigens (molecular weight ranging from 10 000 to 100 000) obtained from one (mono-PV) or three (tri-PV) selected strains. Mono-PV was prepared by the method of ultrafiltration and tri-PV--by differential centrifugtion. Both types of vaccines proved effective in active mouse protection tests after challenge with homologous as well as with heterologous P. aeruginosa strains. Mono-PV is supposed to induce species-specific immunity against P. aeruginosa infection in mice.
已经对几种抗铜绿假单胞菌疫苗进行了实验研究。脓免疫原是一种多组分疫苗，主要由分子量为20000至60000的热稳定蛋白质（或多肽）组成，使用三株或五株选定的铜绿假单胞菌菌株，通过实验室和生产技术制备而成。通过这两种方法获得的制剂毒性低，能在小鼠和大鼠中诱导针对同源和一些异源铜绿假单胞菌菌株（包括产毒PA - 103菌株）攻击的主动免疫。铜绿假单胞菌疫苗（PV）由从一株（单组分PV）或三株（三组分PV）选定菌株中获得的部分纯化的水溶性蛋白质抗原（分子量范围为10000至100000）制备而成。单组分PV通过超滤法制备，三组分PV通过差速离心法制备。在同源和异源铜绿假单胞菌菌株攻击后的主动小鼠保护试验中，这两种疫苗都被证明是有效的。单组分PV被认为能诱导小鼠对铜绿假单胞菌感染产生种特异性免疫。
