使用金胺O对植物细胞中的核DNA进行细胞荧光测定。
Cytofluorometric determination of nuclear DNA in plant cells using auramine O.
作者信息
Cíhalíková J, Dolezel J, Novák F J
出版信息
Acta Histochem. 1985;76(2):151-6. doi: 10.1016/S0065-1281(85)80052-0.
PMID:3929543
Abstract
The effect of DNA hydrolysis, staining time, dye concentration and some other important factors was evaluated on measurement of nuclear DNA content in plant cells by cytofluorometry. Based on the results optimal conditions for making permanent Auramine O--SO2 stained squash preparations can be chosen.
摘要
通过细胞荧光测定法评估了DNA水解、染色时间、染料浓度及其他一些重要因素对植物细胞核DNA含量测量的影响。基于这些结果，可以选择制备永久性金胺O-SO2染色压片标本的最佳条件。
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