Dolezel J
Czechoslovak Academy of Sciences, Institute of Experimental Botany, Olomouc, CSSR.
Acta Histochem. 1989;86(2):123-7. doi: 10.1016/S0065-1281(89)80079-0.
The effect of optical errors and stain variation on the accuracy of plant Feulgen-DNA absorption cytophotometry was investigated. Optical errors (glare, distributional error, and diffraction) were shown to cause important errors. A correction procedure of DUIJNDAM et al. (1980a) was successfully applied to eliminate them. Stain variation can also lead to serious errors and in case different samples are to be compared the use of an internal standard is imperative. Chicken red blood cells proved to be a reliable standard.
研究了光学误差和染色变化对植物福尔根 - DNA吸收细胞光度法准确性的影响。结果表明，光学误差（眩光、分布误差和衍射）会导致重大误差。成功应用了DUIJNDAM等人（1980a）的校正程序来消除这些误差。染色变化也可能导致严重误差，在比较不同样本的情况下，必须使用内标。事实证明，鸡红细胞是一种可靠的标准。
