Dufer J
Acta Histochem. 1985;76(2):219-23. doi: 10.1016/S0065-1281(85)80062-3.
A simple peroxidase staining method specific for eosinophils using 4-chloro-1-naphthol as substrate and 1,2,4-benzene triol as selective inhibitor is applied to the analysis of eosinophil colonies grown in soft-agar cultures form human blood or bone marrow. Eosinophil colonies appear strongly stained in black whereas other types of colonies (neutrophils and macrophages) remain colourless. The proposed method is easy to be performed, quick, reliable, may be associated without major problems to many other cytochemical stains and promises to be of value in hemopoiesis studies.
一种以4-氯-1-萘酚为底物、1,2,4-苯三酚为选择性抑制剂的针对嗜酸性粒细胞的简单过氧化物酶染色方法，被应用于对源自人血液或骨髓的软琼脂培养物中生长的嗜酸性粒细胞集落的分析。嗜酸性粒细胞集落呈现强烈的黑色染色，而其他类型的集落（中性粒细胞和巨噬细胞）则保持无色。所提出的方法易于操作、快速、可靠，与许多其他细胞化学染色联合应用时可能不会出现重大问题，有望在造血研究中具有价值。
