Zucker-Franklin D, Grusky G
J Histochem Cytochem. 1976 Dec;24(12):1270-2. doi: 10.1177/24.12.63511.
There has been a need to easily quantitate the incidence of eosinophil colonies within soft agar cultures. This has been realized by layering of the agar with benzidine dihydrochloride that permits detection of peroxidase activity in cells. Eosinophil colonies can be specifically identified by the addition to the substrate of potassium cyanide, an inhibitor of enzyme activity in neutrophils and monocytes. The enumeration of eosinophil colonies can be accomplished by scanning fresh or embedded cultures with low power magnification.
一直以来都需要能够轻松地对软琼脂培养物中嗜酸性粒细胞集落的发生率进行定量。通过在琼脂中加入盐酸联苯胺来实现这一点，盐酸联苯胺可检测细胞中的过氧化物酶活性。通过向底物中加入氰化钾（一种中性粒细胞和单核细胞中酶活性的抑制剂），可以特异性地识别嗜酸性粒细胞集落。嗜酸性粒细胞集落的计数可以通过用低倍放大扫描新鲜或包埋的培养物来完成。
