Goldberg D J, Sabolinski M, Bystryn J C
Arch Dermatol. 1985 Sep;121(9):1137-40. doi: 10.1001/archderm.121.9.1137.
Human skin, the target organ for bullous pemphigoid (BP) antibodies, is thought to be a less sensitive substrate for the indirect immunofluorescence assay of BP antibodies than monkey or guinea pig esophagus. To examine the reasons for this puzzling phenomenon, we compared the titers of BP antibodies obtained when human skin, monkey, and guinea pig esophagus were used as substrates. We found the titers of BP antibodies obtained with human skin from sites commonly involved in BP (flexor arm, flexor thigh, popliteal fossa) were as high and usually higher than those obtained with monkey and guinea pig esophagus. In contrast, much lower titers were obtained with human skin from sites rarely involved in the disease (scalp, face, extensor arm). These findings suggest that human skin as a substrate is at least as sensitive as monkey or guinea pig esophagus for the indirect immunofluorescence assay of BP antibodies when the skin is obtained from regions on the body commonly involved in BP.
人皮肤是大疱性类天疱疮（BP）抗体的靶器官，人们认为它作为BP抗体间接免疫荧光检测的底物，不如猴或豚鼠食管敏感。为了探究这一令人困惑的现象的原因，我们比较了将人皮肤、猴和豚鼠食管用作底物时获得的BP抗体滴度。我们发现，从BP常见受累部位（手臂屈侧、大腿屈侧、腘窝）获取的人皮肤所测得的BP抗体滴度，与用猴和豚鼠食管测得的滴度一样高，且通常更高。相比之下，从很少受累的部位（头皮、面部、手臂伸侧）获取的人皮肤所测得的滴度则低得多。这些发现表明，当从身体上BP常见受累区域获取皮肤时，人皮肤作为底物在BP抗体间接免疫荧光检测中至少与猴或豚鼠食管一样敏感。
