Bystryn J C, Sabolinski M
J Am Acad Dermatol. 1986 Nov;15(5 Pt 1):973-7. doi: 10.1016/s0190-9622(86)70259-4.
The effect of substrate on the result of indirect immunofluorescence tests for intercellular and basement membrane zone antibodies was examined by testing concurrently 288 human sera on the three substrates used most commonly for this test, that is, monkey and guinea pig esophagus and normal human skin. Seventy-five sera had intercellular antibodies and 56 had basement membrane zone antibodies. The substrate influenced the results of 41% of assays for intercellular and 56% of those for basement membrane zone antibodies. Thirty-one percent of intercellular and 38% of basement membrane zone antibodies failed to react to one or more than one substrate, leading to false-negative reactions. In an additional 10% to 12% of sera, the titer of the reaction was influenced by the substrate. These results indicate that the substrate used has a major impact on the result of indirect immunofluorescence tests for antibodies to skin. The best overall single substrate was monkey esophagus, but optimal results were obtained when both monkey and guinea pig esophagus were used.
通过同时检测288份人血清在该试验最常用的三种底物（即猴和豚鼠食管以及正常人皮肤）上的情况，研究了底物对细胞间和基底膜带抗体间接免疫荧光试验结果的影响。75份血清含有细胞间抗体，56份血清含有基底膜带抗体。底物影响了41%的细胞间抗体检测结果和56%的基底膜带抗体检测结果。31%的细胞间抗体和38%的基底膜带抗体对一种或多种底物无反应，导致假阴性反应。在另外10%至12%的血清中，反应滴度受底物影响。这些结果表明，所用底物对皮肤抗体间接免疫荧光试验结果有重大影响。总体最佳的单一底物是猴食管，但同时使用猴和豚鼠食管时可获得最佳结果。
