Wang Rui, Shen Qing
CAS Key Laboratory of Regenerative Biology, Joint School of Life Sciences, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou Medical University, Guangzhou, China; Centre for Regenerative Medicine and Health, Hong Kong Institute of Science & Innovation, Chinese Academy of Sciences, Hong Kong Special Administrative Region; Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, China-New Zealand Joint Laboratory on Biomedicine and Health, CUHK-GIBH Joint Research Laboratory on Stem Cells and Regenerative Medicine, Institute for Stem Cell and Regeneration, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China; University of Chinese Academy of Sciences, Beijing, China.
Guangzhou Baikang Cell Life Science and Technology Company Limited, Guangzhou, China.
Stem Cell Res. 2024 Dec;81:103556. doi: 10.1016/j.scr.2024.103556. Epub 2024 Sep 14.
The RAB4A gene is a member of the largest group in the Ras superfamily of small GTPases, which regulate membrane trafficking. The encoded protein is associated with early endosomes and is involved in sorting and recycling. In this study, we generated induced pluripotent stem cells (iPSC) from a healthy individual by electroporation of peripheral blood mononuclear cells. We generated a RAB4A homozygous knockout human iPSC line via CRISPR/Cas9 gene editing. The iPSCs-RAB4A had a normal karyotype, expressed pluripotency markers, and maintained trilineage differentiation potential.
RAB4A基因是小GTP酶Ras超家族中最大的一组成员，该家族调节膜运输。编码的蛋白质与早期内体相关，参与分选和再循环。在本研究中，我们通过电穿孔外周血单个核细胞从一名健康个体中生成了诱导多能干细胞（iPSC）。我们通过CRISPR/Cas9基因编辑生成了RAB4A纯合敲除的人类iPSC系。iPSCs-RAB4A具有正常的核型，表达多能性标志物，并保持三系分化潜能。
