Han Hyeong-Jun, Seo Hyang-Hee, Han Hyo-Won, Kim Jung-Hyun
Division of Intractable Diseases, Center for Biomedical Sciences, Korea National Institute of Health, Cheongju 28159, Republic of Korea.
Division of Intractable Diseases, Center for Biomedical Sciences, Korea National Institute of Health, Cheongju 28159, Republic of Korea.
Stem Cell Res. 2019 Oct;40:101520. doi: 10.1016/j.scr.2019.101520. Epub 2019 Jul 31.
Toll Like Receptor (TLR) family plays an important role in the activation of innate immunity against pathogens. TLR7 mediates the recognition of single-stranded RNA viruses, such as human immunodeficiency virus, hepatitis C virus, and influenza virus in endosomes. Here, we generated a TLR7 homozygous knockout human induced pluripotent cell (hiPSC) line, hiPSC-TLR7KO-A59, using the CRISPR/Cas9 genome editing method. The hiPSC-TLR7KO-A59 line maintains normal morphology, pluripotency, and differentiation capacity into three germ layers.
Toll样受体（TLR）家族在激活针对病原体的固有免疫中发挥重要作用。TLR7介导在内体中对单链RNA病毒的识别，如人类免疫缺陷病毒、丙型肝炎病毒和流感病毒。在此，我们使用CRISPR/Cas9基因组编辑方法生成了一个TLR7纯合敲除的人诱导多能干细胞（hiPSC）系，即hiPSC-TLR7KO-A59。hiPSC-TLR7KO-A59系保持正常形态、多能性以及向三个胚层分化的能力。
