State Key Laboratory of Digital Medical Engineering, School of Biological Science and Medical Engineering; Department of neurology, affiliated Zhongda Hospital, Southeast University, Nanjing 210096, China; Institute of Stem Cell and Neural Regeneration, School of pharmacy, Nanjing Medical University, Nanjing 211166, China; State Key Laboratory of Reproductive Medicine and Offspring Health, Nanjing Medical University, Nanjing 211166, China.
Institute of Stem Cell and Neural Regeneration, School of pharmacy, Nanjing Medical University, Nanjing 211166, China; State Key Laboratory of Reproductive Medicine and Offspring Health, Nanjing Medical University, Nanjing 211166, China.
Ecotoxicol Environ Saf. 2024 Oct 15;285:117063. doi: 10.1016/j.ecoenv.2024.117063. Epub 2024 Sep 19.
Nanoplastics are ubiquitous in our daily lives, raising concerns about their potential impact on the human brain. Many studies reported that nanoplastics permeate the blood-brain barrier and influence cellular processes in mouse models. However, the neurotoxic effects of ingesting nanoplastics on human brain remain poorly understood. Here, we treated cerebral organoids with polystyrene nanoplastics to model the effects of nanoplastic exposure on human brain. Importantly, we found that mitochondria might be the significant organelles affected by polystyrene nanoplastics using immunostaing and RNA-seq analysis. Subsequently, we observed the increased cell death and decreased cell differentiation in our cerebral organoids. In conclusion, our findings shed insights on the mechanisms underlying the toxicity of nanoplastics on human brain organoids, providing an evaluation system in detection potential environmental toxicity on human brain.
纳米塑料在我们的日常生活中无处不在，这引发了人们对其对人类大脑潜在影响的担忧。许多研究报告称，纳米塑料会穿透血脑屏障，并影响小鼠模型中的细胞过程。然而，摄入纳米塑料对人类大脑的神经毒性影响仍知之甚少。在这里，我们用聚苯乙烯纳米塑料处理脑类器官，以模拟纳米塑料暴露对人类大脑的影响。重要的是，我们发现使用免疫染色和 RNA-seq 分析，线粒体可能是受聚苯乙烯纳米塑料影响的重要细胞器。随后，我们观察到我们的脑类器官中的细胞死亡增加和细胞分化减少。总之，我们的研究结果揭示了纳米塑料对人类脑类器官毒性的潜在机制，为检测潜在的环境毒性对人类大脑提供了一个评估系统。
