Tao Mingfang, Huang Yuting, Xu Tingting, Peng Xitian, Liao Xianqing, Xia Zhenzhen, Zheng Dan, Li Rong, Xu Xiaoyun
Hubei Key Laboratory of Nutritional Quality and Safety of Agro-products, Institute of Agricultural Quality Standards and Detection Technology, Hubei Academy of Agricultural Sciences, Wuhan, People's Republic of China; Key Laboratory of Environment Correlative Dietology (Ministry of Education), College of Food Science and Technology, Huazhong Agricultural University, Wuhan, People's Republic of China.
Key Laboratory of Environment Correlative Dietology (Ministry of Education), College of Food Science and Technology, Huazhong Agricultural University, Wuhan, People's Republic of China.
J Ethnopharmacol. 2025 Jan 30;337(Pt 1):118838. doi: 10.1016/j.jep.2024.118838. Epub 2024 Sep 17.
Mung bean coat has long been known for its wide-ranging health benefits, including antibacterial, anti-inflammatory, and immune-modulatory properties. For many years in China, mung beans have been employed in the therapeutic management of inflammation induced by pathogenic bacteria infection, yet the precise underlying protective mechanisms remain to be comprehensively elucidated.
Given the growing concern over antibiotic resistance, there is a necessity to explore new anti-infective agents. Here, the anti-infective properties of Mung bean coat extract (MBCE) were investigated using a model of Pseudomonas aeruginosa-infected nematodes.
The protective effects of MBCE on Pseudomonas aeruginosa (PA14) infected nematodes were assessed by lifespan assay, reactive oxygen species (ROS) levels, transcriptomics, and Quantitative real-time PCR (qRT-PCR).
MBCE significantly improved the survival rates and reduced ROS levels in infected worms. Transcriptomic profiling disclosed predominant KEGG pathway enrichments in immune responses, energy metabolism processes such as oxidative phosphorylation and the tricarboxylic acid cycle, alongside aging-related neurodegenerative diseases and longevity regulatory pathways like PI3K-AKT, MAPK, mTOR, and FOXO. qRT-PCR validation showed that MBCE upregulated antimicrobial peptides (spp-3, lys-1, lys-7, abf-2, cnc-2, nlp-33, clec-85), gram-negative responses (irg-3, src-2, grd-3, col-179), and mitochondrial function (mev-1) gene expressions, while downregulated insulin signaling-related (age-1, akt-1, akt-2, daf-15) gene expressions. Mutant strains lifespan analysis indicated that the nsy-1, sek-1, pmk-1, daf-2, aak-2, sir-2.1, and skn-1 were necessary for lifespan extension mediated by MBCE under PA14 infection, but not clk-1, isp-1, mev-1, or daf-16.
Collectively, our findings suggested that MBCE increased the survival rates of PA14-infected worms by activating downstream antimicrobial and antioxidant gene expressions through modulation of MAPK, daf-2, aak-2, sir-2.1, and skn-1 pathways. The research underscored the potential of natural plant compounds to strengthen the body's defenses against infections, potentially mitigating harmful ROS levels and improving survival. Additionally, these findings elucidated the mechanisms by which these plant-derived compounds enhance the immune system, implying their potential utility as dietary supplements or as an alternative to conventional antibiotics.
绿豆皮长期以来因其广泛的健康益处而闻名，包括抗菌、抗炎和免疫调节特性。在中国，绿豆多年来一直被用于治疗由病原菌感染引起的炎症，但其确切的潜在保护机制仍有待全面阐明。
鉴于对抗生素耐药性的日益关注，有必要探索新的抗感染药物。在此，使用铜绿假单胞菌感染线虫模型研究了绿豆皮提取物（MBCE）的抗感染特性。
通过寿命测定、活性氧（ROS）水平、转录组学和定量实时聚合酶链反应（qRT-PCR）评估MBCE对铜绿假单胞菌（PA14）感染线虫的保护作用。
MBCE显著提高了感染线虫的存活率并降低了ROS水平。转录组分析揭示了KEGG主要通路在免疫反应、能量代谢过程（如氧化磷酸化和三羧酸循环）以及与衰老相关的神经退行性疾病和长寿调节通路（如PI3K-AKT、MAPK、mTOR和FOXO）中的富集。qRT-PCR验证表明，MBCE上调了抗菌肽（spp-3、lys-1、lys-7、abf-2、cnc-2、nlp-33、clec-85）、革兰氏阴性反应（irg-3、src-2、grd-3、col-179）和线粒体功能（mev-1）基因的表达，同时下调了胰岛素信号相关（age-1、akt-1、akt-2、daf-15）基因的表达。突变株寿命分析表明，nsy-1、sek-1、pmk-1、daf-2、aak-2、sir-2.1和skn-1是MBCE在PA14感染下介导寿命延长所必需的，但clk-1、isp-1、mev-1或daf-16不是。
总体而言，我们的研究结果表明，MBCE通过调节MAPK、daf-2、aak-2、sir-2.1和skn-1通路激活下游抗菌和抗氧化基因表达，从而提高PA14感染线虫的存活率。该研究强调了天然植物化合物增强机体抗感染防御能力、潜在减轻有害ROS水平和提高存活率的潜力。此外，这些发现阐明了这些植物来源化合物增强免疫系统的机制，暗示了它们作为膳食补充剂或替代传统抗生素的潜在用途。
