Early biological effects in outdoor workers exposed to urban air pollution: A systematic review.

Urban outdoor workers (OWs), identified as professionals spending most of their working shifts in an urban environment, are exposed for at least 8 h/day to traffic air pollution, leading to potential health risks. This paper reports the results of a systematic review aimed at identifying the potential health outcomes of exposure to air pollutants for OWs, focusing mainly on police officers, drivers and street vendors. Health outcomes were analysed in terms of early biological effects quantified with specific measured indicators. The main inclusion criterion was the assessment of at least one early biological effect (genetic and epigenetic damage/alterations, inflammation or oxidative stress indicators, or hormonal imbalance) in a population of OWs exposed to urban air pollution. By applying the PRISMA workflow, 82 papers were included in this study. The results showed that the measured pollutant concentrations were significantly below the current occupational limit values, while exceeds the indications of WHO for urban air pollution. This exposure led to significant alterations of biological markers in OWs with respect to non-exposed subjects. In particular, OWs presented an increased frequency of micronuclei and DNA adducts as the main DNA alterations, while police officers (a category of highly exposed OWs) showed hormonal alterations affecting mainly the hypothalamic-pituitary-gonadal axis. Concerning oxidative stress and inflammation, all the analysed matrices (i.e. blood, sputum, urine and lachrymal fluids) showed increased indices for OWs respect to non-exposed groups. Therefore, the evaluation of effect biomarkers to detect early alterations provides crucial information for supporting the occupational risk management of OWs and, at broader level, allows for an insight of the early-stage health outcomes due to urban air pollution.