Aliabadi Elham, Amooei Fateme, Khozani Tahereh Talaei, Karbalay-Doust Saied, Karimi Fatemeh
Anatomy Department, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
Tissue Engineering Lab, Anatomy Department, Shiraz University of Medical Sciences, Shiraz, Iran.
Clin Exp Reprod Med. 2024 Sep 20. doi: 10.5653/cerm.2024.07017.
Ciprofloxacin (CPFX) is frequently prescribed by fertility specialists and urologists to manage infections in male reproductive organs. However, it is toxic to the testicles and can lead to infertility. Dietary antioxidants are known to protect the testis from damage. This study aimed to investigate the effects of coenzyme Q10 (CoQ10) on the adverse side effects of CPFX using stereological methods.
Sixty rats were divided into six groups: control (distilled water), CoQ10 (10 mg/kg/day), and low-dose (103 mg/kg/day) and high-dose (206 mg/kg/day) of CPFX (LD-CPFX, HD-CPFX) with or without CoQ10 consumption. The treatments lasted for 45 days. Sperm count, serum testosterone levels, and testicular parameters were evaluated.
Significant decreases in sperm count, motility, normal morphology, viability, and testosterone levels were observed in the LD-CPFX (p<0.003) and HD-CPFX- treated rats (p=0.0001) compared to the control groups. A 10% to 36% reduction in the volume of seminiferous tubules, tubular epithelium, and tubule length was noted in LD-CPFX (p<0.01) and HD-CPFX-treated rats (p<0.006), while the volume of the interstitium increased by 25% to 28% in LD-CPFX (p=0.03) and HD-CPFX (p=0.008) groups. The number of cells, including spermatogonia, spermatocytes, spermatids, Sertoli cells, and Leydig cells, decreased by 36% to 75% in the testes exposed to LD-CPFX (p<0.04) and HD-CPFX (p<0.01), compared to the control groups. However, these changes normalized in rats that received CoQ10.
CPFX exposure for 45 days, regardless of the dose, has detrimental effects on testicular parameters. CoQ10 can prevent CPFX-induced testicular structural impairments.
环丙沙星（CPFX）常用于生育专家和泌尿科医生治疗男性生殖器官感染。然而，它对睾丸有毒性，可导致不育。已知膳食抗氧化剂可保护睾丸免受损伤。本研究旨在采用体视学方法研究辅酶Q10（CoQ10）对CPFX不良反应的影响。
将60只大鼠分为六组：对照组（蒸馏水）、CoQ10组（10毫克/千克/天），以及低剂量（103毫克/千克/天）和高剂量（206毫克/千克/天）CPFX组（低剂量CPFX组、高剂量CPFX组），每组再分为服用或不服用CoQ10的亚组。治疗持续45天。评估精子计数、血清睾酮水平和睾丸参数。
与对照组相比，低剂量CPFX组（p<0.003）和高剂量CPFX组（p=0.0001）大鼠的精子计数、活力、正常形态、存活率和睾酮水平显著降低。低剂量CPFX组（p<0.01）和高剂量CPFX组（p<0.006）大鼠的生精小管体积、小管上皮和小管长度减少了10%至36%，而低剂量CPFX组（p=0.03）和高剂量CPFX组（p=0.008）间质体积增加了25%至28%。与对照组相比，暴露于低剂量CPFX组（p<0.04）和高剂量CPFX组（p<0.01）的睾丸中，包括精原细胞、精母细胞、精子细胞、支持细胞和间质细胞在内的细胞数量减少了36%至75%。然而，在服用CoQ10的大鼠中，这些变化恢复正常。
无论剂量如何，暴露于CPFX 45天对睾丸参数都有不利影响。CoQ10可以预防CPFX引起的睾丸结构损伤。
