Infectious Disease Responses Laboratory, Prince of Wales Clinical Research Sciences, Sydney, New South Wales, Australia.
JEOL USA Inc, Peabody, Massachusetts, USA.
J Mass Spectrom. 2024 Oct;59(10):e5089. doi: 10.1002/jms.5089.
A peptide's fractional mass is directly associated with its elemental composition and thus amino acid composition. Here it is demonstrated that a peptide's fractional mass alone can be a useful identifier or indicator of that composition for small to mid-sized peptides (5-7 amino acids) and can significantly reduce the number of viable amino acid compositions for larger peptides (> 8 residues) to include or exclude certain possibilities. Separate consideration of the integer portion of the peptide's mass helps to reduce the number of possibilities where many duplicate fractional mass values are found. Adoption of this fractional mass strategy should aid approaches that are presently employed for peptide identification, including in the use of mass map data to search protein databases for proteomics applications.
肽的分数质量与其元素组成直接相关，因此也与其氨基酸组成直接相关。这里证明，对于中小肽（5-7 个氨基酸），肽的分数质量本身可以作为其组成的有用标识符或指示符，并且可以大大减少较大肽（>8 个残基）的可行氨基酸组成数量，以包括或排除某些可能性。单独考虑肽质量的整数部分有助于减少发现许多重复分数质量值的可能性。采用这种分数质量策略应该有助于目前用于肽鉴定的方法，包括在使用质量图谱数据搜索蛋白质数据库进行蛋白质组学应用时。
