Tai Hua-Chia, Checco James W, Sweedler Jonathan V
Department of Chemistry, Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign, Urbana, IL, USA.
Methods Mol Biol. 2018;1719:107-118. doi: 10.1007/978-1-4939-7537-2_7.
D-Amino acid-containing peptides (DAACPs) in animals are a class of bioactive molecules formed via the posttranslational modification of peptides consisting of all-L-amino acid residues. Amino acid residue isomerization greatly impacts the function of the resulting DAACP. However, because isomerization does not change the peptide's mass, this modification is difficult to detect by most mass spectrometry-based peptidomic approaches. Here we describe a method for the identification of DAACPs that can be used to systematically survey peptides extracted from a tissue sample in a non-targeted manner.
动物体内含D-氨基酸的肽（DAACPs）是一类生物活性分子，由全L-氨基酸残基组成的肽经翻译后修饰形成。氨基酸残基异构化对所得DAACP的功能有很大影响。然而，由于异构化不会改变肽的质量，这种修饰很难通过大多数基于质谱的肽组学方法检测到。在这里，我们描述了一种鉴定DAACPs的方法，该方法可用于以非靶向方式系统地检测从组织样本中提取的肽。
