Sobels F H
Environ Mutagen. 1985;7(5):759-73. doi: 10.1002/em.2860070513.
This paper presents a review of various collaborative studies in comparative mutagenesis. The following studies are briefly described: (1) the chemical mutagenesis programme of the European Community, (2) Drosophila studies with various alkylating agents of different s (Swain-Scott) factors, (3) the evaluation by the International Commission for Protection against Environmental Mutagens and Carcinogens (ICPEMC) Committee 1, (4) the Environmental Protection Agency's Gene-Tox Programme, (5) the first and second United Kingdom Environmental Mutagen Society (UKEMS) collaborative studies, and (6) the International Programme on Chemical Safety (IPCS) collaborative study on in vitro tests. The need for chemical dosimetry is emphasized. One of the main conclusions is that, of the mammalian point mutation assays, the L5178Y (TFTR, trifluorothymidine resistant) system showed greatest detection capability in the second UKEMS study. The consensus conclusion of the IPCS in vitro study was that chromosomal aberrations are considered to be the optimal assay for complementing the Salmonella assay and offer the additional advantage that aneuploidy, polyploidy, and sister chromatid exchanges can also be easily assessed.
本文对比较诱变方面的各种合作研究进行了综述。简要描述了以下研究：（1）欧洲共同体的化学诱变计划；（2）使用具有不同s（斯温-斯科特）因子的各种烷化剂对果蝇进行的研究；（3）国际环境诱变剂和致癌物防护委员会（ICPEMC）第1委员会的评估；（4）美国环境保护局的基因毒性计划；（5）英国环境诱变剂学会（UKEMS）的首次和第二次合作研究；以及（6）国际化学品安全计划（IPCS）关于体外试验的合作研究。强调了化学剂量测定的必要性。主要结论之一是，在哺乳动物点突变试验中，L5178Y（TFTR，三氟胸苷抗性）系统在UKEMS的第二项研究中显示出最大的检测能力。IPCS体外研究的共识结论是，染色体畸变被认为是补充沙门氏菌试验的最佳试验，并且具有额外的优势，即非整倍体、多倍体和姐妹染色单体交换也可以很容易地评估。
