de Pauw-Gillet M C, Hennet J J, Bassleer R J
Eur J Cancer Clin Oncol. 1985 Aug;21(8):951-6. doi: 10.1016/0277-5379(85)90114-2.
A spontaneous cyclic phenomenon characterized by successive waves of either high proliferative rate or intense melanogenesis is described in non-confluent B16 melanoma cells subcultivated during 2 months (or more). Dopaoxidase activity is quantified in individual cells after L-dopa reaction, by an original method of visible light absorption cytophotometry. A 24-hr treatment with alpha-MSH increases dopa-oxidase activity. This increase is also noted during the following 14 hr, in a fresh medium devoid of alpha-MSH, in which cell proliferation resumes after 24 hr. Phenylthiourea, cycloheximide or actinomycin D inhibit dopaoxidase activity, but also cell proliferation in alpha-MSH pre-stimulated cells. The effects of the two latter agents suggest that de novo synthesis of the enzyme takes place following alpha-MSH treatment.
在连续传代培养2个月（或更长时间）的未汇合B16黑色素瘤细胞中，描述了一种自发的周期性现象，其特征是相继出现高增殖率或强烈黑色素生成的波浪。在L-多巴反应后，通过一种可见光吸收细胞光度法的原始方法对单个细胞中的多巴氧化酶活性进行定量。用α-MSH进行24小时处理可增加多巴氧化酶活性。在接下来的14小时内，在不含α-MSH的新鲜培养基中也观察到这种增加，在该培养基中细胞增殖在24小时后恢复。苯硫脲、环己酰亚胺或放线菌素D抑制多巴氧化酶活性，但也抑制α-MSH预刺激细胞中的细胞增殖。后两种试剂的作用表明，酶的从头合成在α-MSH处理后发生。
