人肝脏、肠道和肾脏中胆汁酸的葡萄糖醛酸化。猪去氧胆酸的体外研究。
Glucuronidation of bile acids in human liver, intestine and kidney. An in vitro study on hyodeoxycholic acid.
作者信息
Parquet M, Pessah M, Sacquet E, Salvat C, Raizman A, Infante R
出版信息
FEBS Lett. 1985 Sep 23;189(2):183-7. doi: 10.1016/0014-5793(85)81020-6.
The activities of UDP-glucuronyl transferase(s) in homogenates and microsomal preparations of human liver, kidney and intestine were tested with hyodeoxycholic acid (HDC). The various kinetic parameters of the UDC-glucuronidation were determined from time course experiments. In both liver and kidney preparations, HDC underwent a very active metabolic transformation: liver Km = 78 microM, Vmax = 3.3 nmol . min-1 . mg-1 protein; kidney Km = 186 microM, Vmax = 9.9 nmol . min-1 . mg-1 protein. To our knowledge this is the first observation of both an extensive and comparable bile acid glucuronidation occurring in renal and hepatic tissues.
用人肝脏、肾脏和肠道的匀浆及微粒体制剂，以猪去氧胆酸（HDC）检测UDP - 葡萄糖醛酸转移酶的活性。从时程实验确定了UDP - 葡萄糖醛酸化的各种动力学参数。在肝脏和肾脏制剂中，HDC都经历了非常活跃的代谢转化：肝脏的Km = 78微摩尔，Vmax = 3.3纳摩尔·分钟⁻¹·毫克⁻¹蛋白质；肾脏的Km = 186微摩尔，Vmax = 9.9纳摩尔·分钟⁻¹·毫克⁻¹蛋白质。据我们所知，这是首次观察到在肾脏和肝脏组织中发生广泛且相当的胆汁酸葡萄糖醛酸化。
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