Jilin Ginseng Academy, Changchun University of Chinese Medicine, Changchun, PR China.
College of Pharmacy, Jining Medical University, Rizhao, PR China.
PLoS One. 2024 Sep 20;19(9):e0296424. doi: 10.1371/journal.pone.0296424. eCollection 2024.
In this study, we investigated the neuroprotective effect of a water extract of ginseng (WEG) obtained via low-temperature extraction of the brain of mice with Parkinson's disease (PD) and the ameliorative effect on the damaged intestinal system for the treatment of dyskinesia in PD mice. MPTP (1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine) was injected intraperitoneally into male C57BL/6 mice to establish a PD model, and WEG was given via oral gavage. The results indicated that WEG could protect the damaged neuronal cells of the mice brain, inhibit the aggregation of α-synuclein (α-Syn) in the brain, and increase the positive expression rate of tyrosine hydroxylase (TH). WEG significantly improved intestinal damage and regulated intestinal disorders (P<0.05). WEG intervention increased the levels of beneficial bacteria, such as Lactobacillus, and normalized the abundance and diversity of colonies in the intestine of mice. Our results suggested that WEG protected neurons in the brain of PD mice via inhibiting the aggregation of α-Syn in the brain and increasing the positive expression level of TH in the brain. WEG regulated the gut microbiota of mice, improved the behavioral disorders of PD mice, and offered some therapeutic effects on PD mice.
在这项研究中，我们研究了一种通过低温提取帕金森病（PD）小鼠脑获得的人参水提取物（WEG）的神经保护作用，以及对受损肠道系统的改善作用，以治疗 PD 小鼠的运动障碍。通过腹腔注射 1-甲基-4-苯基-1,2,3,6-四氢吡啶（MPTP）建立雄性 C57BL/6 小鼠 PD 模型，并通过口服灌胃给予 WEG。结果表明，WEG 可保护小鼠脑损伤的神经元细胞，抑制脑内α-突触核蛋白（α-Syn）的聚集，并增加酪氨酸羟化酶（TH）的阳性表达率。WEG 显著改善了肠道损伤和肠道紊乱（P<0.05）。WEG 干预增加了有益细菌的水平，如乳酸菌，并使小鼠肠道中的细菌丰度和多样性正常化。我们的结果表明，WEG 通过抑制脑内α-Syn 的聚集和增加脑内 TH 的阳性表达水平来保护 PD 小鼠的神经元。WEG 调节了小鼠的肠道微生物群，改善了 PD 小鼠的行为障碍，并对 PD 小鼠具有一定的治疗效果。
