基于代谢组学启发的发现和组合生物合成的真菌 - 糖基化联苯的多样化。
Metabologenomics-Inspired Discovery and Combinatorial Biosynthesis-Based Diversification of Fungal -Glycosylated Depsides.
机构信息
Key Laboratory of Chemical Biology (Ministry of Education), Shandong Basic Science Research Center (Pharmacy), School of Pharmaceutical Sciences, Cheeloo College of Medicine, Shandong University, Jinan 250012, China.
CAS Key Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou 510301, China.
出版信息
Org Lett. 2024 Oct 4;26(39):8317-8322. doi: 10.1021/acs.orglett.4c03024. Epub 2024 Sep 20.
Through metabologenomics mining, we prioritized SDU 039, a deep-sea sediment-derived fungus producing -glycosylated depsides (-), including seven new species with varying aliphatic chains. Heterologous expression validated the gene cluster, and enzyme assays elucidated the function of glycosyltransferase ExoC. The chemical diversity of -glycosylated depsides is expanded by combinatorial biosynthesis using homologues depside biosynthetic genes and transformation with ExoC and different sugars as substrate.
通过代谢组学挖掘，我们确定了 SDU039 为优先级，这是一种从深海沉积物中分离出来的真菌，能够产生β-糖苷化的联苯二酚（-），其中包括七个具有不同脂肪链的新种。异源表达验证了该基因簇的功能，酶活性分析阐明了糖基转移酶 ExoC 的功能。通过使用同源联苯二酚生物合成基因组合生物合成以及用 ExoC 和不同糖作为底物进行转化，扩展了β-糖苷化联苯二酚的化学多样性。
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