Lavery M, Kearns M J, Price D G, Emery A N, Jefferis R, Nienow A W
Dev Biol Stand. 1985;60:199-206.
Due to the nature of animal cell suspension culture, physical conditions during growth such as pH, pO2, pCO2, agitation and aeration are inter-related and can exhibit individual or combined effects on cells. Evaluation of their influence on hybridoma growth and monoclonal antibody production can only be achieved by the use of well-instrumented stirred tank reactors. To determine the effect of the various physical conditions, mouse hybridomas, excreting antibody specific for human IgG, have been cultured in a 2 litre mechanically agitated round bottomed glass vessel, equipped with facilities to monitor and control these parameters during cell growth. Batch culture has been carried out at 36 degrees C in medium supplemented with 5% foetal calf serum (FCS). Clones have also been grown successfully with 2% FCS additions and in serum free medium, after careful adaptation periods. At least two clones have been assessed for comparative purposes.
由于动物细胞悬浮培养的特性，生长过程中的物理条件，如pH值、pO2、pCO2、搅拌和通气，是相互关联的，并且可能对细胞产生单独或综合的影响。只有使用配备完善的搅拌罐反应器，才能评估它们对杂交瘤生长和单克隆抗体产生的影响。为了确定各种物理条件的影响，已将分泌针对人IgG的特异性抗体的小鼠杂交瘤细胞，在一个2升的机械搅拌圆底玻璃容器中进行培养，该容器配备了在细胞生长过程中监测和控制这些参数的设施。在补充有5%胎牛血清（FCS）的培养基中，于36℃进行分批培养。经过仔细的适应期后，克隆也已在添加2% FCS的培养基和无血清培养基中成功生长。为了进行比较，至少评估了两个克隆。
