MacLeod A J, Thomson M B
Dev Biol Stand. 1985;60:55-61.
Fractions produced during routine production of human plasma protein concentrates using cold ethanol precipitation have been tested for their ability to support animal cell growth in vitro. A mouse-mouse hybridoma cell line, ES-6, which secretes a monoclonal antibody (mab) to human group A red blood cell antigen, has been used as a model. Fraction 4-1 protein has been found to be effective at 0.5-1.0 g/l in RPMI 1640 and has supported growth of ES-6 for 18 months, both growth and mab. production being comparable with that achieved in 10% foetal calf serum. Fraction 4-1 protein has been shown to contain albumin, transferrin, alpha-1 antitrypsin and immunoglobulin. It is reported to be a source of somatomedin C. The immunoglobulin content can be reduced. Other mouse and rat hybridomas, human mab. producing lymphoblastoid cells, Namalva and HEP-2 cells have grown well in medium supplemented with Fraction 4-1 protein.
对使用冷乙醇沉淀法常规生产人血浆蛋白浓缩物过程中产生的各组分，进行了体外支持动物细胞生长能力的测试。一种分泌抗人A血型红细胞抗原单克隆抗体（mab）的小鼠 - 小鼠杂交瘤细胞系ES - 6被用作模型。已发现4 - 1组分蛋白在RPMI 1640培养基中浓度为0.5 - 1.0 g / l时有效，且支持ES - 6生长达18个月，细胞生长和mab产生与在10%胎牛血清中所达到的水平相当。4 - 1组分蛋白已显示含有白蛋白、转铁蛋白、α - 1抗胰蛋白酶和免疫球蛋白。据报道它是生长调节素C的一个来源。其免疫球蛋白含量可以降低。其他小鼠和大鼠杂交瘤、产生人mab的淋巴母细胞样细胞、Namalva细胞和HEP - 2细胞在补充了4 - 1组分蛋白的培养基中生长良好。
