Chen Xiaoqiang, Tang Jianbing, Dong Yunqing, Xuan Min, Tian Yan, Liu Yijie, Peng Na, Cheng Biao
Department of Burn and Plastic Surgery, Southern Theater General Hospital, Guangzhou 510010, China.
Department of Emergency Surgery, Southern Theater General Hospital, Guangzhou 510010, China.
Colloids Surf B Biointerfaces. 2025 Jan;245:114250. doi: 10.1016/j.colsurfb.2024.114250. Epub 2024 Sep 16.
The skin is the immune system's first line of defense. Extensive skin burns can lead to tissue necrosis, sepsis, and even death. Anti-infectious care of burn wounds is a major challenge in clinical medicine. However, the extensive use of antibiotics led to the emergence of multi-drug-resistant bacteria and silver dressings with antibacterial effects are also cytotoxic. We used the natural cationic antibacterial agent ε-polylysine (EPL) to graft Epigallocatechin-3-gallate (EGCG) to synthesize EPL-EGCG. Then, we used methacrylated gelatin (GelMA) with arginine-glycine-aspartate-rich acid (RGD) sequence and EPL-EGCG form interpenetrating polymer network hydrogels with excellent swelling properties. The hydrogel's inherent antibacterial properties and photo-cross-linking properties can cover irregular burn wounds and isolate bacteria to prevent infection. In addition, we used polydopamine (PDA) to coat GelMA microspheres with excellent hemostatic efficacy and load platelet-rich plasma (PRP) to enhance the hemostatic efficacy of the microspheres and impart inflammation-regulating functions. The hydrogel showed excellent hemostatic efficacy in rat liver injury and tail vein injury models. In the rat infected burn model, the hydrogel exhibited favorable antimicrobial, pro-angiogenic, and anti-inflammatory phenotype polarization of macrophages. Our study shows that GelMA/EPL-EGCG/GM-PDA@PRP hydrogel application has excellent antibacterial, hemostatic and anti-inflammatory effects, providing a new treatment strategy for wound care before burn skin grafting.
皮肤是免疫系统的第一道防线。大面积皮肤烧伤可导致组织坏死、败血症甚至死亡。烧伤创面的抗感染护理是临床医学中的一项重大挑战。然而，抗生素的广泛使用导致了多重耐药菌的出现，且具有抗菌作用的银敷料也具有细胞毒性。我们使用天然阳离子抗菌剂ε-聚赖氨酸（EPL）接枝表没食子儿茶素-3-没食子酸酯（EGCG）合成了EPL-EGCG。然后，我们将具有富含精氨酸-甘氨酸-天冬氨酸（RGD）序列的甲基丙烯酸化明胶（GelMA）与EPL-EGCG形成具有优异溶胀性能的互穿聚合物网络水凝胶。该水凝胶固有的抗菌性能和光交联性能能够覆盖不规则的烧伤创面并隔离细菌以防止感染。此外，我们使用聚多巴胺（PDA）包覆具有优异止血效果的GelMA微球并负载富血小板血浆（PRP），以增强微球的止血效果并赋予其炎症调节功能。该水凝胶在大鼠肝损伤和尾静脉损伤模型中显示出优异的止血效果。在大鼠感染性烧伤模型中，该水凝胶表现出良好的抗菌、促血管生成以及巨噬细胞抗炎表型极化作用。我们的研究表明，GelMA/EPL-EGCG/GM-PDA@PRP水凝胶的应用具有优异的抗菌、止血和抗炎效果，为烧伤皮肤移植前的伤口护理提供了一种新的治疗策略。
