Ding Qiang, Mo Zhendong, Wang Xinyue, Chen Meiling, Zhou Fan, Liu Zhengquan, Long Ying, Xia Xianzhu, Zhao Pingsen
Department of Laboratory Medicine, Yuebei People's Hospital Affiliated to Shantou University School of Medicine, Shaoguan 512025, China; Laboratory for Diagnosis of Clinical Microbiology and Infection, Yuebei People's Hospital Affiliated to Shantou University School of Medicine, Shaoguan 512025, China; Shaoguan Municipal Quality Control Center for Laboratory Medicine, Yuebei People's Hospital Affiliated to Shantou University School of Medicine, Shaoguan 512025, China; Shaoguan Municipal Quality Control Center for Surveillance of Bacterial Resistance, Shaoguan 512025, China; Shaoguan Engineering Research Center for Research and Development of Molecular and Cellular Technology in Rapid Diagnosis of Infectious Diseases and Cancer, Shaoguan 512025, China.
Department of Biomedical Engineering, College of Life Science and Technology, Jinan University, Guangzhou 510632, China.
Int J Biol Macromol. 2024 May;266(Pt 1):131257. doi: 10.1016/j.ijbiomac.2024.131257. Epub 2024 Mar 29.
The infected wounds pose one of the major threats to human health today. To address this issue, it is necessary to develop innovative wound dressings with superior antibacterial activity and other properties. Due to its potent antibacterial, antioxidant, and immune-boosting properties, epigallocatechin gallate (EGCG) has been widely utilized. In this study, a multifunctional curdlan hydrogel loading EGCG (Cur-EGCGH3) was designed. Cur-EGCGH3 exhibited excellent physicochemical properties, good biocompatibility, hemostatic, antibacterial, and antioxidant activities. Also, ELISA data showed that Cur-EGCGH3 stimulated macrophages to secrete pro-inflammatory and pro-regenerative cytokines. Cell scratch results indicated that Cur-EGCGH3 promoted the migration of NIH3T3 and HUVECs. In vivo experiments confirmed that Cur-EGCGH3 could inhibit bacterial infection of the infected wounds, accelerate hemostasis, and promote epithelial regeneration and collagen deposition. These results demonstrated that Cur-EGCGH3 holds promise for promoting healing of the infected wounds.
感染伤口是当今人类健康面临的主要威胁之一。为解决这一问题，有必要开发具有卓越抗菌活性及其他特性的创新型伤口敷料。由于表没食子儿茶素没食子酸酯（EGCG）具有强大的抗菌、抗氧化和增强免疫力的特性，已被广泛应用。在本研究中，设计了一种负载EGCG的多功能凝胶多糖水凝胶（Cur-EGCGH3）。Cur-EGCGH3表现出优异的物理化学性质、良好的生物相容性、止血、抗菌和抗氧化活性。此外，酶联免疫吸附测定（ELISA）数据表明，Cur-EGCGH3刺激巨噬细胞分泌促炎和促再生细胞因子。细胞划痕结果表明，Cur-EGCGH3促进了NIH3T3细胞和人脐静脉内皮细胞（HUVECs）的迁移。体内实验证实，Cur-EGCGH3可抑制感染伤口的细菌感染，加速止血，并促进上皮再生和胶原蛋白沉积。这些结果表明，Cur-EGCGH3在促进感染伤口愈合方面具有潜力。
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