Tian Lu, Liu Heng, Zhou Yue, Zhang Chenggui, Bi Ziying, Wu Ting
Department of Histology and Embryology, Dali University, Dali, China; Yunnan Provincial Key Laboratory of Entomological Biopharmaceutical R&D, Dali University, Dali, China.
Yunnan Provincial Key Laboratory of Entomological Biopharmaceutical R&D, Dali University, Dali, China; National-Local Joint Engineering Research Center of Entomoceutics, Dali, Yunnan, China.
Tissue Cell. 2024 Dec;91:102561. doi: 10.1016/j.tice.2024.102561. Epub 2024 Sep 13.
This study aimed to investigate effect of Periplaneta americana extract CII-3 (CII-3) in senescence of SKOV3 cells. Proliferation, colony forming and cell senescence of SKOV3 cells were determined. ROS production was evaluated by flow cytometry. Transcription of telomerase (TERT), p38 MAPK and p53 gene and protein expression of p-p38 MAPK and p-p53, were identified. CII-3 at different concentrations significantly inhibited SKOV3 proliferation, and 80 μg/ml demonstrated the highest inhibitory effect. CII-3 significantly blocked cell cycle in G0/G1 phase (P<0.01) and reduced colony forming efficiency (P<0.001) of SKOV3 cells compared to those in Control group. CII-3 significantly increased SA-β-Gal positive staining SKOV3 cells (P<0.001) and reduced mitochondrial membrane potential (P<0.01) compared to those in Control group. CII-3 markedly decreased TERT gene transcription of SKOV3 cells compared to that in Control group (P<0.001). CII-3 also triggered significantly higher ROS levels in SKOV3 cells compared to that in Control group (P<0.001). CII-3 significantly increased p-p38 MAPK (P<0.001), p-p53 (P<0.001) and p21 (P<0.001) expressions of SKOV3 cells compared to those in Control group. In conclusion, CII-3 triggered cell senescence of SKOV3 cells through activating ROS-p38 MAPK-p53 signaling pathway. This study would provide a promising strategy for inhibiting cancer cell proliferation by including cell senescence.
本研究旨在探讨美洲大蠊提取物CII-3（CII-3）对SKOV3细胞衰老的影响。测定了SKOV3细胞的增殖、集落形成和细胞衰老情况。通过流式细胞术评估活性氧（ROS）的产生。鉴定了端粒酶（TERT）、p38丝裂原活化蛋白激酶（MAPK）和p53基因的转录以及p-p38 MAPK和p-p53的蛋白表达。不同浓度的CII-3均能显著抑制SKOV3细胞的增殖，其中80μg/ml的抑制效果最佳。与对照组相比，CII-3显著阻断SKOV3细胞在G0/G1期的细胞周期（P<0.01），并降低其集落形成效率（P<0.001）。与对照组相比，CII-3显著增加SA-β-Gal阳性染色的SKOV3细胞（P<0.001），并降低线粒体膜电位（P<0.01）。与对照组相比，CII-3显著降低SKOV3细胞的TERT基因转录（P<0.001）。与对照组相比，CII-3还能显著提高SKOV3细胞中的ROS水平（P<0.001）。与对照组相比，CII-3显著增加SKOV3细胞中p-p38 MAPK（P<0.001）、p-p53（P<0.
