念珠菌血症的特异性和非特异性血培养瓶诊断的实验研究。

Experimental study of specific and nonspecific blood culture bottles for the diagnosis of candidemia.

机构信息

Programa de Pós-Graduação em Ciências da Saúde, Faculdade de Medicina (FAMED), Universidade Federal do Rio Grande (FURG), Rio Grande, Rio Grande do Sul (RS), Brazil; Mycology Laboratory of FAMED-FURG, Rio Grande, Rio Grande do Sul (RS), Brazil.

Universidade Federal de Ciências da Saúde de Porto Alegre (UFCSPA), Porto Alegre, Rio Grande do Sul (RS), Brazil.

出版信息

Rev Iberoam Micol. 2024 Jan-Mar;41(1):13-16. doi: 10.1016/j.riam.2024.06.001. Epub 2024 Sep 19.

DOI:10.1016/j.riam.2024.06.001

PMID:39304432

Abstract

BACKGROUND

Early diagnosis of candidemia is critical for the correct management and treatment of patients.

AIMS

To test the efficacy of different blood culture bottles in the growth of Candida strains.

METHODS

We compared the performance of BD BACTEC™ Plus Aerobic/F (Aero) culture bottles with the specific BD BACTEC™ Mycosis IC/F Lytic (Myco) culture bottles using the BD BACTEC™ FX 40 automated blood culture system to determine the mean time-to-detection (TTD) in Candida species. One isolate each of six Candida species was inoculated into blood culture bottles (final concentration, 1-5CFUml) and incubated at 37°C until automated growth detection.

RESULTS

Candida albicans and Nakaseomyces glabratus (Candida glabrata) were detected earlier in the specific culture bottle, whereas Candida tropicalis was detected earlier in the nonspecific bottle; Candida parapsilosis, Pichia kudriavzevii (Candida krusei), and Meyerozyma guilliermondii (Candida guilliermondii) presented similar TTD in both bottles.

CONCLUSIONS

Our study suggests the suitability of using both bottles in clinical laboratories for a faster diagnosis and prompt starting of any treatment.

摘要

背景

早期诊断念珠菌血症对于正确管理和治疗患者至关重要。

目的

测试不同血培养瓶在培养念珠菌菌株方面的效果。

方法

我们使用 BD BACTEC™ FX 40 自动化血液培养系统比较了 BD BACTEC™ Plus Aerobic/F（Aero）培养瓶与特定的 BD BACTEC™ Mycosis IC/F Lytic（Myco）培养瓶的性能，以确定念珠菌属种的平均检测时间（TTD）。将六种念珠菌属的每种一株菌接种到血培养瓶中（终浓度为 1-5CFUml），并在 37°C 孵育直至自动检测到生长。