Friedrich-Loeffler-Institut, Institut für Bakterielle Infektionen und Zoonosen (IBIZ), Naumburger Str. 96a, 07743, Jena, Germany.
Jena University Hospital - Friedrich Schiller University, Institute for Infectious Diseases and Infection Control, Jena, Germany.
Sci Data. 2024 Sep 20;11(1):1030. doi: 10.1038/s41597-024-03818-8.
Tritrichomonas foetus is a parasitic protist responsible for bovine trichomonosis, a reproductive disease associated with significant economic burden to the livestock industry throughout the world. Here, we present a chromosome-level reference genome of T. foetus -KV-1 (ATCC 30924) using short-read (Illumina Miseq), long-read (Oxford Nanopore) and chromatin-linked (Hi-C) sequencing. This is the first chromosome-level genome of a parasitic protist of the order Tritrichomonadida and the second within the Parabasalia lineage, after Trichomonas vaginalis, the human-associated causative agent of the sexually transmitted infection in humans. Our constructed genome is 148 Mb in size, with a N50 length of the scaffolds of 22.9 Mb. The contigs are anchored in five super-scaffolds, corresponding to the expected five chromosomes of the species and covering 78% of the genome assembly. We predict 41,341 protein-coding genes, of which 95.10% have been functionally annotated. This high-quality genome assembly serves as a valuable reference genome for T. foetus to support future studies in functional genomics, genetic conservation and taxonomy.
胎儿三毛滴虫是一种寄生原生动物，可引起牛毛滴虫病，这是一种在世界范围内给畜牧业带来巨大经济负担的生殖疾病。在此，我们利用短读长（Illumina Miseq）、长读长（Oxford Nanopore）和染色质连接（Hi-C）测序技术，展示了胎儿三毛滴虫 -KV-1（ATCC 30924）的染色体水平参考基因组。这是该目寄生原生动物的第一个染色体水平基因组，也是 Parabasalia 谱系中的第二个，仅次于阴道毛滴虫，后者是导致人类性传播感染的人类相关病原体。我们构建的基因组大小为 148Mb，支架 N50 长度为 22.9Mb。这些连续片段被锚定在五个超级支架上，与该物种的预期五条染色体相对应，覆盖了基因组组装的 78%。我们预测了 41341 个编码蛋白的基因，其中 95.10%具有功能注释。这个高质量的基因组组装为胎儿三毛滴虫提供了一个有价值的参考基因组，支持未来在功能基因组学、遗传保护和分类学方面的研究。
