美国怀俄明州、南达科他州和蒙大拿州公牛中的胎儿三毛滴虫临床分离株。
Clinical isolates of Tritrichomonas foetus in bulls in Wyoming, South Dakota and Montana, USA.
机构信息
Department of Veterinary Sciences, University of Wyoming, Laramie, WY, 82070, USA.
Present Address: Division of Pharmacoepidemiology and Pharmacoeconomics, Department of Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, MA, 02115, USA.
出版信息
BMC Vet Res. 2020 Jan 10;16(1):12. doi: 10.1186/s12917-020-2229-6.
BACKGROUND
Several Tritrichomonas species have been found in mammalian hosts. Among these trichomonads T. foetus is often found in the urogenital tract of cattle and the gastrointestinal tract of the domestic cat, resulting in sexually transmitted bovine trichomonosis and fecal-orally transmitted feline trichomonosis, respectively. The aims of the current study were to molecularly characterize clinical isolates of T. foetus in cattle populations in Wyoming, South Dakota, and Montana of the United States of America and to phylogenetically analyze Tritrichomonas species of mammalian hosts.
RESULTS
DNA sequencing of rRNA genes showed over 99% identity of the newly described isolates to other bovine isolates. Further, T. foetus isolates of various mammalian hosts originated in different geographic regions worldwide were clustered into two well-defined clades by phylogenetic analysis of rRNA and cysteine protease 2 genes. Clade I consisted of isolates originated from cattle, pig, and human whereas clade II contained isolates of cat and dog.
CONCLUSION
It is concluded that all mammalian Tritrichomonas spp. apparently belong to T. foetus. Analysis of more sequences is warranted to support this conclusion.
背景
已在哺乳动物宿主中发现了几种滴虫属物种。在这些滴虫中，胎儿滴虫通常存在于牛的泌尿生殖道和家猫的胃肠道中，分别导致性传播的牛滴虫病和粪-口传播的猫滴虫病。本研究的目的是对美国怀俄明州、南达科他州和蒙大拿州的牛群中的胎儿滴虫临床分离株进行分子特征描述，并对哺乳动物宿主的滴虫属物种进行系统发育分析。
结果
rRNA 基因的 DNA 测序显示，新描述的分离株与其他牛分离株的相似度超过 99%。此外，来自不同地理区域的各种哺乳动物宿主的胎儿滴虫分离株通过 rRNA 和半胱氨酸蛋白酶 2 基因的系统发育分析聚集成两个明确的分支。I 分支包含来自牛、猪和人类的分离株，而 II 分支包含来自猫和狗的分离株。
结论
可以得出结论，所有哺乳动物的滴虫属物种显然都属于胎儿滴虫。需要分析更多的序列来支持这一结论。
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